Literature DB >> 9593168

Properties of mutant SHV-5 beta-lactamases constructed by substitution of isoleucine or valine for methionine at position 69.

P Giakkoupi1, V Miriagou, M Gazouli, E Tzelepi, N J Legakis, L S Tzouvelekis.   

Abstract

The effect of replacement of Met-69 by Ile or Val on the properties of the extended-spectrum beta-lactamase SHV-5 was studied. Mutant enzymes were constructed by site-specific mutagenesis and expressed under isogenic conditions in Escherichia coli DH5alpha cells. Compared with SHV-5, the mutant beta-lactamases conferred lower levels of beta-lactam resistance and were less efficient in hydrolyzing ampicillin, cephalothin, and cefotaxime. The substitutions rendered SHV-5 less susceptible to inhibition by clavulanate, sulbactam, and tazobactam; however, the MICs of penicillin-inhibitor combinations remained similar, suggesting an attenuation of penicillinase activity.

Entities:  

Mesh：

Substances：

Year:  1998        PMID： 9593168      PMCID： PMC105805          DOI： 10.1128/AAC.42.5.1281

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  21 in total

1.  OHIO-1 beta-lactamase resistant to mechanism-based inactivators.

Authors:  R A Bonomo; C Currie-McCumber; D M Shlaes
Journal:  FEMS Microbiol Lett       Date:  1992-04-01       Impact factor: 2.742

2.  Nucleotide sequence of the SHV-5 beta-lactamase gene of a Klebsiella pneumoniae plasmid.

Authors:  D Billot-Klein; L Gutmann; E Collatz
Journal:  Antimicrob Agents Chemother       Date:  1990-12       Impact factor: 5.191

3.  A standard numbering scheme for the class A beta-lactamases.

Authors:  R P Ambler; A F Coulson; J M Frère; J M Ghuysen; B Joris; M Forsman; R C Levesque; G Tiraby; S G Waley
Journal:  Biochem J       Date:  1991-05-15       Impact factor: 3.857

4.  Construction and characterization of an OHIO-1 beta-lactamase bearing Met69Ile and Gly238Ser mutations.

Authors:  R A Bonomo; J R Knox; S D Rudin; D M Shlaes
Journal:  Antimicrob Agents Chemother       Date:  1997-09       Impact factor: 5.191

5.  Role of Ser-238 and Lys-240 in the hydrolysis of third-generation cephalosporins by SHV-type beta-lactamases probed by site-directed mutagenesis and three-dimensional modeling.

Authors:  A Huletsky; J R Knox; R C Levesque
Journal:  J Biol Chem       Date:  1993-02-15       Impact factor: 5.157

6.  Characterization of a new TEM-type beta-lactamase resistant to clavulanate, sulbactam, and tazobactam in a clinical isolate of Escherichia coli.

Authors:  J Blazquez; M R Baquero; R Canton; I Alos; F Baquero
Journal:  Antimicrob Agents Chemother       Date:  1993-10       Impact factor: 5.191

7.  Emergence of clinical isolates of Escherichia coli producing TEM-1 derivatives or an OXA-1 beta-lactamase conferring resistance to beta-lactamase inhibitors.

Authors:  X Y Zhou; F Bordon; D Sirot; M D Kitzis; L Gutmann
Journal:  Antimicrob Agents Chemother       Date:  1994-05       Impact factor: 5.191

8.  A general method of site-specific mutagenesis using a modification of the Thermus aquaticus polymerase chain reaction.

Authors:  R M Nelson; G L Long
Journal:  Anal Biochem       Date:  1989-07       Impact factor: 3.365

9.  Site-directed mutagenesis at the active site of Escherichia coli TEM-1 beta-lactamase. Suicide inhibitor-resistant mutants reveal the role of arginine 244 and methionine 69 in catalysis.

Authors:  M Delaire; R Labia; J P Samama; J M Masson
Journal:  J Biol Chem       Date:  1992-10-15       Impact factor: 5.157

10.  Characterization of a beta-lactamase from Clostridium clostridioforme.

Authors:  P C Appelbaum; S K Spangler; G A Pankuch; A Philippon; M R Jacobs; R Shiman; E J Goldstein; D M Citron
Journal:  J Antimicrob Chemother       Date:  1994-01       Impact factor: 5.790
View more
  1 in total

1.  Construction and characterization of mutants of the TEM-1 beta-lactamase containing amino acid substitutions associated with both extended-spectrum resistance and resistance to beta-lactamase inhibitors.

Authors:  P D Stapleton; K P Shannon; G L French
Journal:  Antimicrob Agents Chemother       Date:  1999-08       Impact factor: 5.191
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.