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Literature DB >> 9592770

PIG-B: a homemade monophasic cocktail for the extraction of RNA.

Abstract

An inexpensive monophasic reagent has been developed for the extraction of total RNA from cells or tissues. The main ingredients of the reagent are Phenol, Isoamyl alcohol, Guanidinium isothiocyanate, and Beta-mercaptoethanol (PIG-B). The quality and yield of RNA obtained by this reagent is at par with that obtained by TRIzol, an expensive but widely used monophasic reagent available commercially. The complete composition and method of preparation of PIG-B is provided to aid preparation of the reagent in the laboratory.

Entities: Chemical Gene

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Year: 1998 PMID： 9592770 DOI： 10.1007/BF02752699

Source DB: PubMed Journal: Mol Biotechnol ISSN： 1073-6085 Impact factor: 2.695

5 in total

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Authors: K S KIRBY
Journal: Biochem J Date: 1956-11 Impact factor: 3.857

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Authors: P Chomczynski
Journal: Biotechniques Date: 1993-09 Impact factor: 1.993

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Authors: G Sarkar; S S Sommer
Journal: Science Date: 1989-04-21 Impact factor: 47.728

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Authors: P Chomczynski; N Sacchi
Journal: Anal Biochem Date: 1987-04 Impact factor: 3.365

5. The "looped oligo" method for generating reference molecules for quantitative PCR.

Authors: G Sarkar; M E Bolander
Journal: Biotechniques Date: 1994-11 Impact factor: 1.993

5 in total

23 in total

1. Repeat cDNA synthesis and RT-PCR with the same source of RNA.

Authors: B Fuchs; K Zhang; M G Rock; M E Bolander; G Sarkar
Journal: Mol Biotechnol Date: 1999-10 Impact factor: 2.695

2. High temperature cDNA synthesis by AMV reverse transcriptase improves the specificity of PCR.

Authors: B Fuchs; K Zhang; M G Rock; M E Bolander; G Sarkar
Journal: Mol Biotechnol Date: 1999-10 Impact factor: 2.695

3. A method for accurate detection of translocation junctions in Ewing family of tumors.

Authors: T Morishita; M E Bolander; K Zhang; S Tamai; Y Mii; G Sarkar
Journal: Mol Biotechnol Date: 2001-06 Impact factor: 2.695

4. Extraction of total RNA from a high pigment content plant. Marigold (Tagetes erecta).

Authors: B Chi-Manzanero; M L Robert; R Rivera-Madrid
Journal: Mol Biotechnol Date: 2000-09 Impact factor: 2.695

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6. Naturally arising strains of polyomaviruses with severely attenuated microRNA expression.

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7. Insights into Polyomaviridae microRNA function derived from study of the bandicoot papillomatosis carcinomatosis viruses.

Authors: Chun Jung Chen; Rodney P Kincaid; Gil Ju Seo; Mark D Bennett; Christopher S Sullivan
Journal: J Virol Date: 2011-02-23 Impact factor: 5.103