A E Chauvet1, P P Kesava, C S Goh, B Badie. 1. Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, USA.
Abstract
OBJECT: The goal of this study was to evaluate gene delivery to a benign brain tumor. METHODS: A recombinant adenovirus vector bearing the Escherichia coli beta-galactosidase reporter gene was selectively injected into the vascular supply of a spontaneously occurring canine olfactory groove meningioma. The tumor and a small amount of peritumoral brain tissue were removed 5 days after viral injection and stained with X-Gal to assess gene delivery. The authors noted significant beta-galactosidase gene expression by the tumor, but not by surrounding brain tissue. No obvious viral-related cytotoxicity was noted. CONCLUSIONS: The authors found that meningiomas can be successfully transduced by adenovirus vectors by using endovascular techniques.
OBJECT: The goal of this study was to evaluate gene delivery to a benign brain tumor. METHODS: A recombinant adenovirus vector bearing the Escherichia colibeta-galactosidase reporter gene was selectively injected into the vascular supply of a spontaneously occurring canine olfactory groove meningioma. The tumor and a small amount of peritumoral brain tissue were removed 5 days after viral injection and stained with X-Gal to assess gene delivery. The authors noted significant beta-galactosidase gene expression by the tumor, but not by surrounding brain tissue. No obvious viral-related cytotoxicity was noted. CONCLUSIONS: The authors found that meningiomas can be successfully transduced by adenovirus vectors by using endovascular techniques.
Authors: Marianela Candolfi; Kurt M Kroeger; G Elizabeth Pluhar; Josee Bergeron; Mariana Puntel; James F Curtin; Elizabeth A McNiel; Andrew B Freese; John R Ohlfest; Peter Moore; Pedro R Lowenstein; Maria G Castro Journal: Neurosurgery Date: 2007-01 Impact factor: 4.654
Authors: Michael R Olin; G Elizabeth Pluhar; Brian M Andersen; Rob Shaver; Nate N Waldron; Christopher L Moertel Journal: Crit Rev Immunol Date: 2014 Impact factor: 2.214
Authors: W K de Roos; J H de Wilt; M E van Der Kaaden; E R Manusama; M W de Vries; A Bout; T L ten Hagen; D Valerio; A M Eggermont Journal: Ann Surg Date: 2000-12 Impact factor: 12.969
Authors: Seunguk Oh; G Elizabeth Pluhar; Elizabeth A McNeil; Kurt M Kroeger; Chunyan Liu; Maria G Castro; Pedro R Lowenstein; Andrew Freese; John R Ohlfest Journal: J Neurosurg Date: 2007-07 Impact factor: 5.115