Literature DB >> 9573172

Mutational analysis of the phosphate-binding loop of Rhizobium meliloti DctD, a sigma54-dependent activator.

Y Gao1, Y K Wang, T R Hoover.   

Abstract

The phosphate-binding loop of sigma54-dependent activators is thought to participate in ATP binding and/or hydrolysis. Alanine substitutions at positions 3, 4, 6, 7, and 8 of this motif in Rhizobium meliloti DctD disrupted transcriptional activation and ATP hydrolysis. Interestingly, substitution of alanine at position 7 also affected DNA binding.

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Year:  1998        PMID： 9573172      PMCID： PMC107239          DOI： 10.1128/JB.180.10.2792-2795.1998

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  29 in total

1.  ATP binding to the sigma 54-dependent activator XylR triggers a protein multimerization cycle catalyzed by UAS DNA.

Authors:  J Pérez-Martín; V de Lorenzo
Journal:  Cell       Date:  1996-07-26       Impact factor: 41.582

2.  A conserved region in the sigma54-dependent activator DctD is involved in both binding to RNA polymerase and coupling ATP hydrolysis to activation.

Authors:  Y K Wang; J H Lee; J M Brewer; T R Hoover
Journal:  Mol Microbiol       Date:  1997-10       Impact factor: 3.501

3.  Active site comparisons highlight structural similarities between myosin and other P-loop proteins.

Authors:  C A Smith; I Rayment
Journal:  Biophys J       Date:  1996-04       Impact factor: 4.033

4.  Alterations within the activation domain of the sigma 54-dependent activator DctD that prevent transcriptional activation.

Authors:  Y K Wang; T R Hoover
Journal:  J Bacteriol       Date:  1997-09       Impact factor: 3.490

5.  Transcriptional activation of the Klebsiella pneumoniae nitrogenase promoter may involve DNA loop formation.

Authors:  M Buck; W Cannon; J Woodcock
Journal:  Mol Microbiol       Date:  1987-09       Impact factor: 3.501

6.  Protein crosslinking studies suggest that Rhizobium meliloti C4-dicarboxylic acid transport protein D, a sigma 54-dependent transcriptional activator, interacts with sigma 54 and the beta subunit of RNA polymerase.

Authors:  J H Lee; T R Hoover
Journal:  Proc Natl Acad Sci U S A       Date:  1995-10-10       Impact factor: 11.205

7.  Transcriptional activation via DNA-looping: visualization of intermediates in the activation pathway of E. coli RNA polymerase x sigma 54 holoenzyme by scanning force microscopy.

Authors:  K Rippe; M Guthold; P H von Hippel; C Bustamante
Journal:  J Mol Biol       Date:  1997-07-11       Impact factor: 5.469

8.  Cooperative binding of DctD to the dctA upstream activation sequence of Rhizobium meliloti is enhanced in a constitutively active truncated mutant.

Authors:  D Scholl; B T Nixon
Journal:  J Biol Chem       Date:  1996-10-18       Impact factor: 5.157

9.  Deduced products of C4-dicarboxylate transport regulatory genes of Rhizobium leguminosarum are homologous to nitrogen regulatory gene products.

Authors:  C W Ronson; P M Astwood; B T Nixon; F M Ausubel
Journal:  Nucleic Acids Res       Date:  1987-10-12       Impact factor: 16.971

10.  Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.

Authors:  J E Walker; M Saraste; M J Runswick; N J Gay
Journal:  EMBO J       Date:  1982       Impact factor: 11.598
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  5 in total

1.  Mutations affecting motifs of unknown function in the central domain of nitrogen regulatory protein C.

Authors:  J Li; L Passaglia; I Rombel; D Yan; S Kustu
Journal:  J Bacteriol       Date:  1999-09       Impact factor: 3.490

2.  Purification and characterization of the AAA+ domain of Sinorhizobium meliloti DctD, a sigma54-dependent transcriptional activator.

Authors:  Hao Xu; Baohua Gu; B Tracy Nixon; Timothy R Hoover
Journal:  J Bacteriol       Date:  2004-06       Impact factor: 3.490

3.  prpR, ntrA, and ihf functions are required for expression of the prpBCDE operon, encoding enzymes that catabolize propionate in Salmonella enterica serovar typhimurium LT2.

Authors:  S Palacios; J C Escalante-Semerena
Journal:  J Bacteriol       Date:  2000-02       Impact factor: 3.490

4.  MgATP binding and hydrolysis determinants of NtrC, a bacterial enhancer-binding protein.

Authors:  I Rombel; P Peters-Wendisch; A Mesecar; T Thorgeirsson; Y K Shin; S Kustu
Journal:  J Bacteriol       Date:  1999-08       Impact factor: 3.490

5.  Regulation of the transcriptional activator NtrC1: structural studies of the regulatory and AAA+ ATPase domains.

Authors:  Seok-Yong Lee; Armando De La Torre; Dalai Yan; Sydney Kustu; B Tracy Nixon; David E Wemmer
Journal:  Genes Dev       Date:  2003-10-15       Impact factor: 11.361
  5 in total

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