| Literature DB >> 956665 |
T Ahern, G A Taylor, C J Sanderson.
Abstract
Different grades of glass fibre filter paper have been compared for harvesting cells labelled with tritium using a semi-automatic harvester. Grade GF/C (Whatman Ltd) was selected for routine use although GF/B was found to be useful for whole blood cultures. The factors affecting the choice of thymidine concentration and activity are discussed and 1 mjCi or [3H]thymidine (5 Ci/mMol)/0.2 ml culture is shown to give a linear relationship with total DNA synthesis for up to 22 h.Entities:
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Year: 1976 PMID： 956665 DOI： 10.1016/0022-1759(76)90027-2
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303