BACKGROUND: An antiendomysium antibody test using human umbilical cord as antigen has recently been introduced. METHODS: We determined IgA- and IgG-class antihuman umbilical cord (HUC-ab), antireticulin (ARA), and antigliadin antibodies (AGA) in 92 untreated adult coeliac patients, in 95 non-coeliac subjects, and in 4 coeliac patients with selective IgA deficiency. Tissue antibodies were measured with an indirect immunofluorescence method and AGA with an enzyme-linked immunosorbent assay. RESULTS: Of adult coeliac patients 85% were positive for IgA-class HUC-ab, 78% were positive for ARA, and 80% for AGA; the specificity for HUC-ab and ARA was 100%, and for AGA 86%. Combination of HUC-ab, ARA, and high-titre AGA increased the sensitivity to 96% without loss of specificity. IgG-class HUC-ab was positive in 12% of coeliac patients, in all four coeliac patients with IgA deficiency, and in none of the controls. CONCLUSIONS: The HUC-ab test is highly specific but not 100% sensitive for detecting adult coeliac disease. A combination of the IgA-class HUC-ab, ARA, and high-titre AGA tests is recommended. In selective IgA deficiency the IgG-class HUC-ab test seems to work well.
BACKGROUND: An antiendomysium antibody test using human umbilical cord as antigen has recently been introduced. METHODS: We determined IgA- and IgG-class antihuman umbilical cord (HUC-ab), antireticulin (ARA), and antigliadin antibodies (AGA) in 92 untreated adult coeliac patients, in 95 non-coeliac subjects, and in 4 coeliac patients with selective IgA deficiency. Tissue antibodies were measured with an indirect immunofluorescence method and AGA with an enzyme-linked immunosorbent assay. RESULTS: Of adult coeliac patients 85% were positive for IgA-class HUC-ab, 78% were positive for ARA, and 80% for AGA; the specificity for HUC-ab and ARA was 100%, and for AGA 86%. Combination of HUC-ab, ARA, and high-titre AGA increased the sensitivity to 96% without loss of specificity. IgG-class HUC-ab was positive in 12% of coeliac patients, in all four coeliac patients with IgA deficiency, and in none of the controls. CONCLUSIONS: The HUC-ab test is highly specific but not 100% sensitive for detecting adult coeliac disease. A combination of the IgA-class HUC-ab, ARA, and high-titre AGA tests is recommended. In selective IgA deficiency the IgG-class HUC-ab test seems to work well.
Authors: V Baldas; A Tommasini; C Trevisiol; I Berti; A Fasano; D Sblattero; A Bradbury; R Marzari; G Barillari; A Ventura; T Not Journal: Gut Date: 2000-11 Impact factor: 23.059
Authors: I R Korponay-Szabó; I Dahlbom; K Laurila; S Koskinen; N Woolley; J Partanen; J B Kovács; M Mäki; T Hansson Journal: Gut Date: 2003-11 Impact factor: 23.059
Authors: Sara Ashorn; Hanna Raukola; Tuuli Välineva; Merja Ashorn; Bo Wei; Jonathan Braun; Immo Rantala; Katri Kaukinen; Tiina Luukkaala; Pekka Collin; Markku Mäki; Sari Iltanen Journal: J Clin Immunol Date: 2008-05-22 Impact factor: 8.317
Authors: Liisa Viitasalo; Laura Niemi; Merja Ashorn; Sara Ashorn; Jonathan Braun; Heini Huhtala; Pekka Collin; Markku Mäki; Katri Kaukinen; Kalle Kurppa; Sari Iltanen Journal: J Clin Gastroenterol Date: 2014-08 Impact factor: 3.062
Authors: Anitta Vilppula; Katri Kaukinen; Liisa Luostarinen; Ilkka Krekelä; Heikki Patrikainen; Raisa Valve; Markku Luostarinen; Kaija Laurila; Markku Mäki; Pekka Collin Journal: BMC Gastroenterol Date: 2011-12-16 Impact factor: 3.067