Literature DB >> 9526659

Development and applications of enhanced green fluorescent protein mutants.

R H Stauber1, K Horie, P Carney, E A Hudson, N I Tarasova, G A Gaitanaris, G N Pavlakis.   

Abstract

The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) fluorescent protein, gave 50-fold-100-fold brighter fluorescence compared to wild-type GFP and BFP (Tyr66His), respectively, upon expression in mammalian cells. GFPsg25 and BFPsg50 have different excitation and emission maxima. This allows their use as an efficient dual-color tagging system and their independent detection in living cells.

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Year:  1998        PMID： 9526659     DOI： 10.2144/98243rr01

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  42 in total

1.  Mechanism for down-regulation of CD28 by Nef.

Authors:  T Swigut; N Shohdy; J Skowronski
Journal:  EMBO J       Date:  2001-04-02       Impact factor: 11.598

2.  A synthetic HIV-1 Rev inhibitor interfering with the CRM1-mediated nuclear export.

Authors:  Dirk Daelemans; Elena Afonina; Jakob Nilsson; Gudrun Werner; Jorgen Kjems; Erik De Clercq; George N Pavlakis; Anne-Mieke Vandamme
Journal:  Proc Natl Acad Sci U S A       Date:  2002-10-09       Impact factor: 11.205

3.  Dribble, the Drosophila KRR1p homologue, is involved in rRNA processing.

Authors:  H Y Chan; S Brogna; C J O'Kane
Journal:  Mol Biol Cell       Date:  2001-05       Impact factor: 4.138

4.  Functional nuclear topography of transcriptionally inducible extra-chromosomal transgene clusters.

Authors:  Manja Meggendorfer; Claudia Weierich; Horst Wolff; Ruth Brack-Werner; Thomas Cremer
Journal:  Chromosome Res       Date:  2010-06-08       Impact factor: 5.239

5.  Integrated functional and bioinformatics approach for the identification and experimental verification of RNA signals: application to HIV-1 INS.

Authors:  Horst Wolff; Ruth Brack-Werner; Markus Neumann; Thomas Werner; Ralf Schneider
Journal:  Nucleic Acids Res       Date:  2003-06-01       Impact factor: 16.971

6.  Structural and functional analysis of the RNA transport element, a member of an extensive family present in the mouse genome.

Authors:  Sergey Smulevitch; Daniel Michalowski; Andrei S Zolotukhin; Ralf Schneider; Jenifer Bear; Patricia Roth; George N Pavlakis; Barbara K Felber
Journal:  J Virol       Date:  2005-02       Impact factor: 5.103

7.  Green fluorescent protein is superior to blue fluorescent protein as a quantitative reporter of promoter activity in E. coli.

Authors:  James L Lissemore; Joshua Bayes; Molly Calvey; Lucas Reineke; Anne Colagiavanni; Melissa Tscheiner; David P Mascotti
Journal:  Mol Biol Rep       Date:  2008-07-13       Impact factor: 2.316

8.  New adenovirus vectors for protein production and gene transfer.

Authors:  B Massie; D D Mosser; M Koutroumanis; I Vitté-Mony; L Lamoureux; F Couture; L Paquet; C Guilbault; J Dionne; D Chahla; P Jolicoeur; Y Langelier
Journal:  Cytotechnology       Date:  1998-11       Impact factor: 2.058

9.  Identification of a heterogeneous nuclear ribonucleoprotein-recognition region in the HIV Rev protein.

Authors:  Kamyar Hadian; Michelle Vincendeau; Nina Mäusbacher; Daniel Nagel; Stefanie M Hauck; Marius Ueffing; Abraham Loyter; Thomas Werner; Horst Wolff; Ruth Brack-Werner
Journal:  J Biol Chem       Date:  2009-10-05       Impact factor: 5.157

10.  Identification of novel import and export signals of human TAP, the protein that binds to the constitutive transport element of the type D retrovirus mRNAs.

Authors:  J Bear; W Tan; A S Zolotukhin; C Tabernero; E A Hudson; B K Felber
Journal:  Mol Cell Biol       Date:  1999-09       Impact factor: 4.272
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