| Literature DB >> 95182 |
K Shortman, D S Linthicum, F L Battye, I Goldschneider, A Liabeuf, P Golstein, E A Clark, P Lake.
Abstract
A number of analytical techniques for distinguishing and separating the "high theta" "low theta" subpopulations of mouse thymocytes have been compared. A differential cytotoxic assay was compared to a quantitative immunofluorescent assay on individual cells using flow cytofluorometry and cell sorting. Conventional anti-Thy-1 antisera were compared with a monoclonal IgM anti-Thy-1. The monoclonal reagent greatly improved both types of assay, eliminated a number of artifacts and allowed either procedure to be used to give a clear distinction, based on Thy-1 level, between the two subpopulations. The distribution of Thy-1 on thymocytes is bimodal, rather than continuous. These separate "high theta" and "low theta" categories each includes a population a population of dividing cells.Entities:
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Year: 1979 PMID： 95182 DOI： 10.1007/bf02783667
Source DB: PubMed Journal: Cell Biophys ISSN： 0163-4992