Literature DB >> 950380

Large-scale purification of hepatitis B surface antigen.

J Vnek, A M Prince.   

Abstract

Hepatitis B surface antigen was concentrated and purified from plasma by two simple steps of purification. In the first step the antigen was purified 24-fold by polyethylene glycol precipitation. An additional 10-fold purification was achieved by batchwise adsorption to hydroxylapatite and subsequent elution with 0.02 M sodium phosphate buffer.

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Year:  1976        PMID： 950380      PMCID： PMC274366          DOI： 10.1128/jcm.3.6.626-631.1976

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

1.  Characterization of hepatitis B antigen polypeptides.

Authors:  C R Howard; A J Zuckerman
Journal:  Intervirology       Date:  1974       Impact factor: 1.763

2.  Affinity chromatography of hepatitis B antigen on concanavalin A linked to sepharose.

Authors:  A R Neurath; A M Prince; A Lippin
Journal:  J Gen Virol       Date:  1973-06       Impact factor: 3.891

3.  Concentration and purification of hepatitis B antigen with polyethylene glycol and polyelectrolyte 60, a cross-linked copolymer of isobutylene maleic anhydride.

Authors:  E De Rizzo; R Pandey; C Wallis; J L Melnick
Journal:  Infect Immun       Date:  1972-09       Impact factor: 3.441

4.  Separation and purification of hepatitis-associated antigen into morphologic types by zonal ultracentrifugation.

Authors:  H E Bond; W T Hall
Journal:  J Infect Dis       Date:  1972-03       Impact factor: 5.226

5.  The use of an immunoadsorbent in the isolation of Australia antigen.

Authors:  B Houwen; J Marrink; J Vos; I Molenaar; H O Nieweg
Journal:  Vox Sang       Date:  1973       Impact factor: 2.144

6.  Isolation and purification of hepatitis-associated antigen by affinity chromatography with baboon antiserum.

Authors:  W O Grabow; O W Prozesky
Journal:  J Infect Dis       Date:  1973-02       Impact factor: 5.226

7.  A simple procedure for purification of hepatitis B-antigen.

Authors:  A Cruceanu; R Müller; H Deicher
Journal:  Med Microbiol Immunol       Date:  1973-09-26       Impact factor: 3.402

8.  The purification of hepatitis-associated antigen (HAA-SH-AU) from human serum and the preparation of a sheep anti-HAA serum.

Authors:  W J Duimel; H G Brummelhuis; H W Krijnen
Journal:  Vox Sang       Date:  1972       Impact factor: 2.144

9.  Production of potent anti-Australia antigen sera of high specificity and sensitivity in goats.

Authors:  G R Dreesman; F B Hollinger; R M McCombs; J L Melnick
Journal:  Infect Immun       Date:  1972-02       Impact factor: 3.441

10.  Australia antigen (a hepatitis-associated antigen): purification and physical properties.

Authors:  I Millman; L A Loeb; M E Bayer; B S Blumberg
Journal:  J Exp Med       Date:  1970-06-01       Impact factor: 14.307
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  3 in total

1.  Heterogeneity of hepatitis B surface antigen-associated particles isolated from chimpanzee plasma.

Authors:  J Vnek; H Ikram; A M Prince
Journal:  Infect Immun       Date:  1977-04       Impact factor: 3.441

2.  Development and technical and clinical validation of a quantitative enzyme-linked immunosorbent assay for the detection of human antibodies to hepatitis B surface antigen in recipients of recombinant hepatitis B virus vaccine.

Authors:  Pierre Cambron; Jeanne-Marie Jacquet; Bernard Hoet; Marc Lievens
Journal:  Clin Vaccine Immunol       Date:  2009-06-24

3.  Rapid and Robust Continuous Purification of High-Titer Hepatitis B Virus for In Vitro and In Vivo Applications.

Authors:  Jochen M Wettengel; Bianca Linden; Knud Esser; Michael Laue; Benjamin J Burwitz; Ulrike Protzer
Journal:  Viruses       Date:  2021-07-30       Impact factor: 5.048
  3 in total

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