Literature DB >> 4118047

Concentration and purification of hepatitis B antigen with polyethylene glycol and polyelectrolyte 60, a cross-linked copolymer of isobutylene maleic anhydride.

E De Rizzo, R Pandey, C Wallis, J L Melnick.   

Abstract

A simple two-step procedure is described for the concentration and purification of hepatitis B antigen. The antigen is precipitated by polyethylene glycol and then adsorbed on and eluted from an insoluble polyelectrolyte, polyelectrolyte 60, which is a cross-linked copolymer of isobutylene maleic anhydride.

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Year:  1972        PMID： 4118047      PMCID： PMC422537          DOI： 10.1128/iai.6.3.335-338.1972

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  13 in total

1.  Stability of the lipid and protein of hepatitis-associated (Australia) antigen.

Authors:  C Y Kim; D M Bissell
Journal:  J Infect Dis       Date:  1971-05       Impact factor: 5.226

2.  Australia antigen: large-scale purification from human serum and biochemical studies of its proteins.

Authors:  J L Gerin; P V Holland; R H Purcell
Journal:  J Virol       Date:  1971-05       Impact factor: 5.103

3.  Biophysical properties of Australia antigen.

Authors:  J L Gerin; R H Purcell; M D Hoggan; P V Holland; R M Chanock
Journal:  J Virol       Date:  1969-11       Impact factor: 5.103

4.  Enhanced detection of Australia antigen in serum hepatitis patients by discontinuous counter-immunoelectrophoresis.

Authors:  C Wallis; J L Melnick
Journal:  Appl Microbiol       Date:  1971-05

5.  RNA of Australia antigen.

Authors:  W Józwiak; J Koscielak; K Madaliński; W J Brzosko; A Nowoslawski; M Kloczewiak
Journal:  Nat New Biol       Date:  1971-01-20

6.  Precipitating heterologous antisera for the detection of Au-SH-antigen.

Authors:  W Becker; L Bonacker
Journal:  Vox Sang       Date:  1970 Sep-Oct       Impact factor: 2.144

7.  Further studies on a "new" human isoprecipitin system (Australia antigen).

Authors:  H J Alter; B S Blumberg
Journal:  Blood       Date:  1966-03       Impact factor: 22.113

8.  Concentration and purification of viruses by adsorption to and elution from insoluble polyelectrolytes.

Authors:  C Wallis; J L Melnick; J E Fields
Journal:  Appl Microbiol       Date:  1971-04

9.  Preparation and standardization of an Australia antigen antibody of equine origin.

Authors:  V J Cabasso; R Nieman; D D Schroeder; K A Hok; R E Louie; M M Mozen
Journal:  Appl Microbiol       Date:  1971-06

10.  Australia antigen (a hepatitis-associated antigen): purification and physical properties.

Authors:  I Millman; L A Loeb; M E Bayer; B S Blumberg
Journal:  J Exp Med       Date:  1970-06-01       Impact factor: 14.307
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  5 in total

1.  Optimal conditions for elution of hepatitis B antigen after absorption onto colloidal silica.

Authors:  J Pillot; S Goueffon; R G Keros
Journal:  J Clin Microbiol       Date:  1976-09       Impact factor: 5.948

2.  Heterogeneity of hepatitis B surface antigen-associated particles isolated from chimpanzee plasma.

Authors:  J Vnek; H Ikram; A M Prince
Journal:  Infect Immun       Date:  1977-04       Impact factor: 3.441

3.  Large-scale purification of hepatitis B surface antigen.

Authors:  J Vnek; A M Prince
Journal:  J Clin Microbiol       Date:  1976-06       Impact factor: 5.948

4.  A simple procedure for purification of hepatitis B-antigen.

Authors:  A Cruceanu; R Müller; H Deicher
Journal:  Med Microbiol Immunol       Date:  1973-09-26       Impact factor: 3.402

5.  Affinity chromatography of hepatitis B surface antigen from e-antigen-positive sera on insolubilized cross-linked albumin. Brief report.

Authors:  A R Neurath; N Strick
Journal:  Arch Virol       Date:  1979       Impact factor: 2.574
  5 in total

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