Literature DB >> 9501457

Expression of psychrophilic genes in mesophilic hosts: assessment of the folding state of a recombinant alpha-amylase.

G Feller1, O Le Bussy, C Gerday.   

Abstract

Alpha-Amylase from the antarctic psychrophile Altermonas haloplanktis is synthesized at 0 +/- 2 degrees C by the wild strain. This heat-labile alpha-amylase folds correctly when overexpressed in Escherichia coli, providing the culture temperature is sufficiently low to avoid irreversible denaturation. In the described expression system, a compromise between enzyme stability and E. coli growth rate is reached at 18 degrees C.

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Year:  1998        PMID： 9501457      PMCID： PMC106386          DOI： 10.1128/AEM.64.3.1163-1165.1998

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  7 in total

1.  Nucleotide and derived amino acid sequence of the subtilisin from the antarctic psychrotroph Bacillus TA39.

Authors:  E Narinx; S Davail; G Feller; C Gerday
Journal:  Biochim Biophys Acta       Date:  1992-05-07

2.  Heat-labile alkaline phosphatase from Antarctic bacteria: Rapid 5' end-labeling of nucleic acids.

Authors:  H Kobori; C W Sullivan; H Shizuya
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

Review 3.  Bacterial life at low temperature: physiological aspects and biotechnological implications.

Authors:  A M Gounot
Journal:  J Appl Bacteriol       Date:  1991-11

4.  Cloning and expression in Escherichia coli of three lipase-encoding genes from the psychrotrophic antarctic strain Moraxella TA144.

Authors:  G Feller; M Thiry; J L Arpigny; C Gerday
Journal:  Gene       Date:  1991-06-15       Impact factor: 3.688

5.  Purification, characterization, and nucleotide sequence of the thermolabile alpha-amylase from the antarctic psychrotroph Alteromonas haloplanctis A23.

Authors:  G Feller; T Lonhienne; C Deroanne; C Libioulle; J Van Beeumen; C Gerday
Journal:  J Biol Chem       Date:  1992-03-15       Impact factor: 5.157

6.  Stability and structural analysis of alpha-amylase from the antarctic psychrophile Alteromonas haloplanctis A23.

Authors:  G Feller; F Payan; F Theys; M Qian; R Haser; C Gerday
Journal:  Eur J Biochem       Date:  1994-06-01

7.  Sequence of the subtilisin-encoding gene from an antarctic psychrotroph Bacillus TA41.

Authors:  S Davail; G Feller; E Narinx; C Gerday
Journal:  Gene       Date:  1992-09-21       Impact factor: 3.688
  7 in total
  14 in total

Review 1.  Search and discovery strategies for biotechnology: the paradigm shift.

Authors:  A T Bull; A C Ward; M Goodfellow
Journal:  Microbiol Mol Biol Rev       Date:  2000-09       Impact factor: 11.056

Review 2.  Molecular basis of cold adaptation.

Authors:  Salvino D'Amico; Paule Claverie; Tony Collins; Daphné Georlette; Emmanuelle Gratia; Anne Hoyoux; Marie-Alice Meuwis; Georges Feller; Charles Gerday
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2002-07-29       Impact factor: 6.237

Review 3.  Atlantic cod trypsins: from basic research to practical applications.

Authors:  Agústa Gudmundsdóttir; Helga Margrét Pálsdóttir
Journal:  Mar Biotechnol (NY)       Date:  2005-02-17       Impact factor: 3.619

4.  Characterization of recombinant glutathione reductase from the psychrophilic Antarctic bacterium Colwellia psychrerythraea.

Authors:  Mikyoung Ji; Callie V Barnwell; Amy M Grunden
Journal:  Extremophiles       Date:  2015-06-23       Impact factor: 2.395

5.  Expression of a temperature-sensitive esterase in a novel chaperone-based Escherichia coli strain.

Authors:  Manuel Ferrer; Tatyana N Chernikova; Kenneth N Timmis; Peter N Golyshin
Journal:  Appl Environ Microbiol       Date:  2004-08       Impact factor: 4.792

6.  Cold-adapted RTX lipase from antarctic Pseudomonas sp. strain AMS8: isolation, molecular modeling and heterologous expression.

Authors:  Mohd Shukuri Mohamad Ali; Menega Ganasen; Raja Noor Zaliha Raja Abd Rahman; Adam Leow Thean Chor; Abu Bakar Salleh; Mahiran Basri
Journal:  Protein J       Date:  2013-04       Impact factor: 2.371

7.  Cold shock induction of recombinant Arctic environmental genes.

Authors:  Gro Elin Kjæreng Bjerga; Adele Kim Williamson
Journal:  BMC Biotechnol       Date:  2015-08-19       Impact factor: 2.563

Review 8.  Marine extremophiles: a source of hydrolases for biotechnological applications.

Authors:  Gabriel Zamith Leal Dalmaso; Davis Ferreira; Alane Beatriz Vermelho
Journal:  Mar Drugs       Date:  2015-04-03       Impact factor: 5.118

Review 9.  Biotechnological uses of enzymes from psychrophiles.

Authors:  R Cavicchioli; T Charlton; H Ertan; S Mohd Omar; K S Siddiqui; T J Williams
Journal:  Microb Biotechnol       Date:  2011-03-24       Impact factor: 5.813

10.  Biotechnology of cold-active proteases.

Authors:  Swati Joshi; Tulasi Satyanarayana
Journal:  Biology (Basel)       Date:  2013-05-03
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