K Z Liu1, T C Dembinski, H H Mantsch. 1. Institute for Biodiagnostics, National Research Council of Canada, Winnipeg, Manitoba.
Abstract
OBJECTIVE: The purpose of this study was to determine the feasibility of infrared spectroscopy as an alternative method for the prediction of fetal lung development. STUDY DESIGN: Samples of amniotic fluid were obtained by amniocentesis from 134 patients between the 26th and 41st week of pregnancy. To acquire infrared spectra with a commercial spectrometer, 35 microl of amniotic fluid was required for each spectrum. The lecithin/sphingomyelin ratio was determined separately by thin-layer chromatography for each sample. A robust linear discriminant analysis method was used to partition the samples into normal and abnormal groups according to their infrared spectra. A partial least squares regression analysis was then applied to quantitatively correlate the infrared spectra with the thin-layer chromatography-based lecithin/sphingomyelin ratios. RESULTS: Qualitatively, the infrared spectra of the amniotic fluid in the validation set were successfully partitioned into lecithin/sphingomyelin <2 and lecithin/sphingomyelin >2 groups. Quantitatively, the lecithin/sphingomyelin ratios predicted from the infrared spectra of amniotic fluid were highly correlated and in excellent agreement with those determined by thin-layer chromatography (correlation coefficient = 0.893). CONCLUSION: Infrared spectroscopy has the potential to become the clinical method of choice for determining fetal lung surfactant maturity in amniotic fluid.
OBJECTIVE: The purpose of this study was to determine the feasibility of infrared spectroscopy as an alternative method for the prediction of fetal lung development. STUDY DESIGN: Samples of amniotic fluid were obtained by amniocentesis from 134 patients between the 26th and 41st week of pregnancy. To acquire infrared spectra with a commercial spectrometer, 35 microl of amniotic fluid was required for each spectrum. The lecithin/sphingomyelin ratio was determined separately by thin-layer chromatography for each sample. A robust linear discriminant analysis method was used to partition the samples into normal and abnormal groups according to their infrared spectra. A partial least squares regression analysis was then applied to quantitatively correlate the infrared spectra with the thin-layer chromatography-based lecithin/sphingomyelin ratios. RESULTS: Qualitatively, the infrared spectra of the amniotic fluid in the validation set were successfully partitioned into lecithin/sphingomyelin <2 and lecithin/sphingomyelin >2 groups. Quantitatively, the lecithin/sphingomyelin ratios predicted from the infrared spectra of amniotic fluid were highly correlated and in excellent agreement with those determined by thin-layer chromatography (correlation coefficient = 0.893). CONCLUSION: Infrared spectroscopy has the potential to become the clinical method of choice for determining fetal lung surfactant maturity in amniotic fluid.
Authors: Henrik Verder; Christian Heiring; Howard Clark; David Sweet; Torben E Jessen; Finn Ebbesen; Lars J Björklund; Bengt Andreasson; Lars Bender; Aksel Bertelsen; Marianne Dahl; Christian Eschen; Jesper Fenger-Grøn; Stine F Hoffmann; Agnar Höskuldsson; Maria Bruusgaard-Mouritsen; Fredrik Lundberg; Anthony D Postle; Peter Schousboe; Peter Schmidt; Hristo Stanchev; Lars Sørensen Journal: Acta Paediatr Date: 2016-12-20 Impact factor: 2.299