Literature DB >> 9497922

High resolution single strand conformation polymorphism analysis using formamide and ethidium bromide staining.

T Xie1, S L Ho, O C Ma.   

Abstract

Single strand conformation polymorphism (SSCP) analysis using ethidium bromide can be improved by adding formamide as the denaturant. This gives higher resolution than previous SSCP methods; it had 100% sensitivity in the discrimination of 14 PCR samples from two different genes, even for a long fragment close to the upper limit of 250 base pairs. This modified procedure is a rapid, simple, safe, and yet highly sensitive method for detecting structural differences in DNA fragments.

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Year:  1997        PMID： 9497922      PMCID： PMC379648          DOI： 10.1136/mp.50.5.276

Source DB:  PubMed          Journal:  Mol Pathol        ISSN： 1366-8714


  5 in total

1.  Nonisotopic SSCP detection in PCR products by ethidium bromide staining.

Authors:  E P Yap; J O McGee
Journal:  Trends Genet       Date:  1992-02       Impact factor: 11.639

2.  Non-isotopic detection of single strand conformation polymorphism (PCR-SSCP): a rapid and sensitive technique in diagnosis of phenylketonuria.

Authors:  B Dockhorn-Dworniczak; B Dworniczak; L Brömmelkamp; J Bülles; J Horst; W W Böcker
Journal:  Nucleic Acids Res       Date:  1991-05-11       Impact factor: 16.971

3.  Diagnostic single strand conformational polymorphism, (SSCP): a simplified non-radioisotopic method as applied to a Tay-Sachs B1 variant.

Authors:  P J Ainsworth; L C Surh; M B Coulter-Mackie
Journal:  Nucleic Acids Res       Date:  1991-01-25       Impact factor: 16.971

4.  Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms.

Authors:  M Orita; H Iwahana; H Kanazawa; K Hayashi; T Sekiya
Journal:  Proc Natl Acad Sci U S A       Date:  1989-04       Impact factor: 11.205

5.  'Cold SSCP': a simple, rapid and non-radioactive method for optimized single-strand conformation polymorphism analyses.

Authors:  T Hongyo; G S Buzard; R J Calvert; C M Weghorst
Journal:  Nucleic Acids Res       Date:  1993-08-11       Impact factor: 16.971
  5 in total
  8 in total

1.  Molecular characterization of antigen B2 subunit in two genotypes of Echinococcus granulosus from Indian bubaline isolates, its stage specific expression and serological evaluation.

Authors:  D Pan; A K Bera; S Bandyopadhyay; S Das; T Rana; S K Das; S Bandyopadhyay; B Manna; D Bhattacharya
Journal:  Mol Biol Rep       Date:  2010-09-18       Impact factor: 2.316

Review 2.  PCR-SSCP: a method for the molecular analysis of genetic diseases.

Authors:  V Konstantinos Kakavas; Kakavas V Konstantinos; Panagiotis Plageras; Plageras Panagiotis; T Antonios Vlachos; Vlachos T Antonios; Agelos Papaioannou; Papaioannou Agelos; V Argiris Noulas; Noulas V Argiris
Journal:  Mol Biotechnol       Date:  2007-10-13       Impact factor: 2.695

3.  A valid mouse model of AGRIN-associated congenital myasthenic syndrome.

Authors:  Laurent P Bogdanik; Robert W Burgess
Journal:  Hum Mol Genet       Date:  2011-09-01       Impact factor: 6.150

4.  Molecular characterization of Echinococcus granulosus of Indian animal isolates on the basis of nuclear and mitochondrial genotype.

Authors:  J Gudewar; D Pan; A K Bera; S K Das; A Konar; J R Rao; A K Tiwari; D Bhattacharya
Journal:  Mol Biol Rep       Date:  2008-07-31       Impact factor: 2.316

5.  Mutation scan screening of Echinococcus granulosus isolates of Indian origin.

Authors:  D Bhattacharya; D Pan; A K Bera; A Konar; S K Das
Journal:  Vet Res Commun       Date:  2008-02-19       Impact factor: 2.459

6.  Polymorphism and natural selection of antigen B1 of Echinococcus granulosus isolated from different host assemblages in India.

Authors:  D Pan; A K Bera; S K Das; S Bandyopadhyay; B Manna; D Bhattacharya
Journal:  Mol Biol Rep       Date:  2009-05-10       Impact factor: 2.316

7.  Evaluation of Bax and Bak gene mutations and expression in breast cancer.

Authors:  Naglaa Mohamed Kholoussi; Sobhy E H El-Nabi; Nora Nassef Esmaiel; Naser Mohamed Abd El-Bary; Ahmed F El-Kased
Journal:  Biomed Res Int       Date:  2014-02-09       Impact factor: 3.411

8.  Characterisation of Early-Life Fecal Microbiota in Susceptible and Healthy Pigs to Post-Weaning Diarrhoea.

Authors:  Samir Dou; Pascale Gadonna-Widehem; Véronique Rome; Dounia Hamoudi; Larbi Rhazi; Lyes Lakhal; Thibaut Larcher; Narges Bahi-Jaber; Arturo Pinon-Quintana; Alain Guyonvarch; Isabelle L E Huërou-Luron; Latifa Abdennebi-Najar
Journal:  PLoS One       Date:  2017-01-10       Impact factor: 3.240
  8 in total

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