Literature DB >> 949338

A sensitive method for measuring protein turnover based on the measurement of 2-3H-labelled amino acids in protein.

T J Humphrey, D D Davies.   

Abstract

A method for measuring the rate of protein degradation is described. The method measures the change in 2-3H content of protein with time by racemization of the protein hydrolysate with acetic anhydride. The 3H on C-2 of amino acids is stable in proteins but becomes labile, owing to the action of transaminases, once the amino acids are released by proteolysis. The specific measurement of 2-3H in amino acids largely overcomes problems due to compartmentation and isotope recycling and evidence to support this claim is presented. Values for the half-life of Lemna minor (duckweed) protein determined by the new method are compared with values obtained by other methods.

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Year:  1976        PMID： 949338      PMCID： PMC1163789          DOI： 10.1042/bj1560561

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  4 in total

1.  A new method for the measurement of protein turnover.

Authors:  T J Humphrey; D D Davies
Journal:  Biochem J       Date:  1975-04       Impact factor: 3.857

2.  The Turnover of Nucleic Acids in Lemna minor.

Authors:  A Trewavas
Journal:  Plant Physiol       Date:  1970-06       Impact factor: 8.340

3.  Protein metabolism in cultured plant tissues. Calculation of an absolute rate of protein synthesis, accumulation, and degradation in tobacco callus in vivo.

Authors:  J D Kemp; D W Sutton
Journal:  Biochemistry       Date:  1971-01-05       Impact factor: 3.162

4.  D2O-alanine exchange reactions catalyzed by alanine racemase and glutamic pyruvic transminase.

Authors:  U M Babu; R B Johnston
Journal:  Biochem Biophys Res Commun       Date:  1974-05-20       Impact factor: 3.575
  4 in total
  17 in total

1.  Amino Acid recycling in relation to protein turnover.

Authors:  D D Davies; T J Humphrey
Journal:  Plant Physiol       Date:  1978-01       Impact factor: 8.340

2.  Model for Stress-induced Protein Degradation in Lemna minor.

Authors:  R J Cooke; K Roberts; D D Davies
Journal:  Plant Physiol       Date:  1980-12       Impact factor: 8.340

3.  The effect of deuterium oxide on protein turnover in Lemna minor.

Authors:  R J Cooke; S Grego; J Oliver; D D Davies
Journal:  Planta       Date:  1979-01       Impact factor: 4.116

4.  The mechanism of deuterium oxide-induced protein degradation in Lemna minor.

Authors:  R J Cooke; S Grego; K Roberts; D D Davies
Journal:  Planta       Date:  1980-04       Impact factor: 4.116

5.  An association between photorespiration and protein catabolism: Studies with Chlamydomonas.

Authors:  J V Cullimore; A P Sims
Journal:  Planta       Date:  1980-12       Impact factor: 4.116

6.  Homocysteine Biosynthesis in Green Plants: Physiological Importance of the Transsulfuration Pathway in Lemna paucicostata.

Authors:  P K Macnicol; A H Datko; J Giovanelli; S H Mudd
Journal:  Plant Physiol       Date:  1981-09       Impact factor: 8.340

7.  Lemna paucicostata Hegelm. 6746: LIFE CYCLE AND CHARACTERIZATION OF THE COLONY TYPES IN A POPULATION.

Authors:  A H Datko; S H Mudd; J Giovanelli
Journal:  Plant Physiol       Date:  1980-05       Impact factor: 8.340

8.  Stress and Protein Turnover in Lemna minor.

Authors:  R J Cooke; J Oliver; D D Davies
Journal:  Plant Physiol       Date:  1979-12       Impact factor: 8.340

9.  Effects of deuterium oxide on growth, proton extrusion, potassium influx, and in vitro plasma membrane activities in maize root segments.

Authors:  G A Sacchi; M Cocucci
Journal:  Plant Physiol       Date:  1992-12       Impact factor: 8.340

10.  The role of glutathione in amino-acid absorption. Lack of correlation between glutathione turnover and amino-acid absorption by the yeast Candida utilis.

Authors:  R J Robins; D D Davies
Journal:  Biochem J       Date:  1981-01-15       Impact factor: 3.857
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