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Literature DB >> 9490804

In vivo mapping of nucleosomes using psoralen-DNA crosslinking and primer extension.

Abstract

By the use of psoralen crosslinking and primer extension, a method was developed which allows the analysis of chromatin structure in vivo. Using a yeast minichromosome, >9 nucleosomes were mapped with a resolution of at least +/-30 bp.

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Year: 1998 PMID： 9490804 PMCID： PMC147415 DOI： 10.1093/nar/26.6.1544

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

8 in total

1. Ribosomal gene clusters are uniquely proportioned between open and closed chromatin structures in both tomato leaf cells and exponentially growing suspension cultures.

Authors: A Conconi; J M Sogo; C A Ryan
Journal: Proc Natl Acad Sci U S A Date: 1992-06-15 Impact factor: 11.205

2. Probes of DNA structure.

Authors: D M Lilley
Journal: Methods Enzymol Date: 1992 Impact factor: 1.600

3. Chromatin structure of the yeast URA3 gene at high resolution provides insight into structure and positioning of nucleosomes in the chromosomal context.

Authors: S Tanaka; M Livingstone-Zatchej; F Thoma
Journal: J Mol Biol Date: 1996-04-19 Impact factor: 5.469

4. Cross-linking of DNA in situ as a probe for chromatin structure.

Authors: C V Hanson; C K Shen; J E Hearst
Journal: Science Date: 1976-07-02 Impact factor: 47.728

5. Electron microscopy of chromatin.

Authors: J M Sogo; F Thoma
Journal: Methods Enzymol Date: 1989 Impact factor: 1.600

6. UV-induced pyrimidine dimers and trimethylpsoralen cross-links do not alter chromatin folding in vitro.

Authors: J M Gale; M J Smerdon
Journal: Biochemistry Date: 1988-09-20 Impact factor: 3.162

7. Protein-DNA interactions and nuclease-sensitive regions determine nucleosome positions on yeast plasmid chromatin.

Authors: F Thoma
Journal: J Mol Biol Date: 1986-07-20 Impact factor: 5.469

8. Psoralen-crosslinking of soluble and of H1-depleted soluble rat liver chromatin.

Authors: A Conconi; R Losa; T Koller; J M Sogo
Journal: J Mol Biol Date: 1984-10-05 Impact factor: 5.469

8 in total

4 in total

In vivo mapping of nucleosomes using psoralen-DNA crosslinking and primer extension.

1. Ribosomal gene clusters are uniquely proportioned between open and closed chromatin structures in both tomato leaf cells and exponentially growing suspension cultures.

2. Probes of DNA structure.

3. Chromatin structure of the yeast URA3 gene at high resolution provides insight into structure and positioning of nucleosomes in the chromosomal context.

4. Cross-linking of DNA in situ as a probe for chromatin structure.

5. Electron microscopy of chromatin.

6. UV-induced pyrimidine dimers and trimethylpsoralen cross-links do not alter chromatin folding in vitro.

7. Protein-DNA interactions and nuclease-sensitive regions determine nucleosome positions on yeast plasmid chromatin.

8. Psoralen-crosslinking of soluble and of H1-depleted soluble rat liver chromatin.

1. Nucleosome positioning at the replication fork.

2. A method for genome-wide analysis of DNA helical tension by means of psoralen-DNA photobinding.

Review 3. Single-Molecule Techniques to Study Chromatin.

4. High-resolution, genome-wide mapping of positive supercoiling in chromosomes.