Literature DB >> 948248

Sensitive fluorometric method for tissue tocopherol analysis.

S L Taylor, M P Lamden, A L Tappel.   

Abstract

A sensitive, highly reproducible method for tissue tocopherol analysis that combines saponification in the presence of large nmount of ascorbic acid to remove interfering substances, extraction fo the nonsponifiable lipids with hexane, and fluorometric measurement of the tocopherol is presented. The nonsaponifiable lipids phase contained only one fluorochrome in the 290 am excitation and 330 nm emission range, and it was identified as tocopherol by thin layer and column chromatography. Column chromatography of the hexane extract of a saponified, 14C-tocopherolspiked microsomal fraction showed that no measurable oxidation to tocopherylquinone had occurred. The flurometric method for tocopherol analysis was applied to homogenates and subcellular fractions from rat liver, kidney, lung, and heart and red blood cells. The heavy mitochondrial and microsomal fractions had the highest subcellular concentrations of tocopherol.

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Year:  1976        PMID： 948248     DOI： 10.1007/bf02532898

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  22 in total

1.  THE ABSORPTION, STORAGE, AND METABOLISM OF ALPHA-TOCOPHEROL-C14 IN THE RAT AND CHICKEN.

Authors:  S KRISHNAMURTHY; J G BIERI
Journal:  J Lipid Res       Date:  1963-07       Impact factor: 5.922

2.  Spectrofluorometric determination of tocopherols.

Authors:  D E DUGGAN
Journal:  Arch Biochem Biophys       Date:  1959-09       Impact factor: 4.013

3.  Studies on Vitamin E. 1. The determination of tocopherols in animal tissues.

Authors:  E E EDWIN; A T DIPLOCK; J BUNYAN; J GREEN
Journal:  Biochem J       Date:  1960-06       Impact factor: 3.857

4.  Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.

Authors:  C DE DUVE; B C PRESSMAN; R GIANETTO; R WATTIAUX; F APPELMANS
Journal:  Biochem J       Date:  1955-08       Impact factor: 3.857

5.  The influence of tocopherols on U.S.P. XIV vitamin A assay.

Authors:  S H FOX; A MUELLER
Journal:  J Am Pharm Assoc Am Pharm Assoc       Date:  1950-11

6.  Identification of alpha-tocopherol from tissues by combined gas-liquid chromatography, mass spectrometry and infrared spectroscopy.

Authors:  P P Nair; Z Luna
Journal:  Arch Biochem Biophys       Date:  1968-09-20       Impact factor: 4.013

7.  A fluorometric micro method for fat tocopherol.

Authors:  L G Hansen; W J Warwick
Journal:  Clin Biochem       Date:  1970-09       Impact factor: 3.281

8.  Accumulation of lipofuscin-like pigment in the rat adrenal gland as a function of vitamin E deficiency.

Authors:  W B Weglicki; W Reichel; P P Nair
Journal:  J Gerontol       Date:  1968-10

9.  Microquantitative separation of isomeric dimethyltocols by gas-liquid chromatography.

Authors:  P P Nair; I Sarlos; J Machiz
Journal:  Arch Biochem Biophys       Date:  1966-06       Impact factor: 4.013

10.  Spectrophotometric method for determination of tocopherol in red blood cells.

Authors:  H J Kayden; C K Chow; L K Bjornson
Journal:  J Lipid Res       Date:  1973-09       Impact factor: 5.922
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  30 in total

1.  A new rotifer-based assay for tocopherol.

Authors:  M E Kabay; J J Gilbert
Journal:  Lipids       Date:  1977-10       Impact factor: 1.880

2.  Protective effects of the glutathione redox cycle and vitamin E on cultured fibroblasts infected by Mycoplasma pneumoniae.

Authors:  M Almagor; I Kahane; C Gilon; S Yatziv
Journal:  Infect Immun       Date:  1986-04       Impact factor: 3.441

3.  Oxidative stress and protective mechanisms in erythrocytes in relation to Plasmodium vinckei load.

Authors:  R Stocker; N H Hunt; G D Buffinton; M J Weidemann; P H Lewis-Hughes; I A Clark
Journal:  Proc Natl Acad Sci U S A       Date:  1985-01       Impact factor: 11.205

4.  Transmural distribution of antioxidant defences and lipid peroxidation in the rabbit left ventricular myocardium.

Authors:  D Lapenna; A Mezzetti; S de Gioia; A Consoli; D Festi; C Di Ilio; F Cuccurullo
Journal:  Pflugers Arch       Date:  1994-07       Impact factor: 3.657

5.  Evaluation of alpha-tocopherol antioxidant activity in microsomal lipid peroxidation as detected by low-level chemiluminescence.

Authors:  E Cadenas; M Ginsberg; U Rabe; H Sies
Journal:  Biochem J       Date:  1984-11-01       Impact factor: 3.857

6.  Study of subcellular localization of Glycine max γ-tocopherol methyl transferase isoforms in N. benthamiana.

Authors:  Khushboo Kumari; Monika Prakash Rai; Navita Bansal; G Rama Prashat; Sweta Kumari; Rohini Srivathsa; Anil Dahuja; Archana Sachdev; Shelly Praveen; T Vinutha
Journal:  3 Biotech       Date:  2020-02-11       Impact factor: 2.406

7.  Rubidium transport in irradiated vitamin-E-deficient bone marrow cells.

Authors:  K I Altman; H Mühlensiepen; R Wolters; O Muzik; L E Feinendegen
Journal:  Radiat Environ Biophys       Date:  1993       Impact factor: 1.925

8.  Lipid peroxidation in rat tissue slices: effect of dietary vitamin E, corn oil-lard and menhaden oil.

Authors:  B E Leibovitz; M L Hu; A L Tappel
Journal:  Lipids       Date:  1990-03       Impact factor: 1.880

9.  The effect of platelet-activating factor on the responsiveness of the human nasal airway.

Authors:  C E Austin; J C Foreman
Journal:  Br J Pharmacol       Date:  1993-09       Impact factor: 8.739

10.  Lipid peroxidation and lysosomal enzymes in D-galactosamine hepatitis and its protection by vitamin E.

Authors:  T Yoshikawa; N Yokoe; S Takemura; H Kato; T Hotta; N Matsumura; M Ikezaki; K Hosokawa; M Kondo
Journal:  Gastroenterol Jpn       Date:  1979
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