Literature DB >> 9466785

Comparison of use of cerebrospinal fluid, serum, and throat swab specimens in diagnosis of enteroviral acute neurological infection by a rapid RNA detection PCR assay.

L Andréoletti1, N Blassel-Damman, A Dewilde, L Vallée, R Cremer, D Hober, P Wattré.   

Abstract

A PCR assay for detection of enterovirus RNA in multiple specimen types from patients with neurological infections was evaluated. Combined PCR assay of cerebrospinal fluid and serum (systemic specimens) was more sensitive than assaying either specimen alone in children but not in adults. Compared with PCR in systemic specimens, detection of enterovirus RNA in throat swabs showed a sensitivity of 62.5% and a specificity of 75.6%.

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Year:  1998        PMID: 9466785      PMCID: PMC104586     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  11 in total

1.  Rapid and sensitive detection of enteroviruses in specimens from patients with aseptic meningitis.

Authors:  S Yerly; A Gervaix; V Simonet; M Caflisch; L Perrin; W Wunderli
Journal:  J Clin Microbiol       Date:  1996-01       Impact factor: 5.948

2.  Diagnosis of enterovirus infection by polymerase chain reaction of multiple specimen types.

Authors:  H A Rotbart; A Ahmed; S Hickey; R Dagan; G H McCracken; R J Whitley; J F Modlin; M Cascino; J F O'Connell; M A Menegus; D Blum
Journal:  Pediatr Infect Dis J       Date:  1997-04       Impact factor: 2.129

3.  Evaluation of an enterovirus group-specific anti-VP1 monoclonal antibody, 5-D8/1, in comparison with neutralization and PCR for rapid identification of enteroviruses in cell culture.

Authors:  A Trabelsi; F Grattard; M Nejmeddine; M Aouni; T Bourlet; B Pozzetto
Journal:  J Clin Microbiol       Date:  1995-09       Impact factor: 5.948

4.  Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay.

Authors:  H A Rotbart; M H Sawyer; S Fast; C Lewinski; N Murphy; E F Keyser; J Spadoro; S Y Kao; M Loeffelholz
Journal:  J Clin Microbiol       Date:  1994-10       Impact factor: 5.948

5.  Rapid detection of enterovirus in clinical specimens using PCR and microwell capture hybridization assay.

Authors:  L Andréoletti; D Hober; S Belaich; P E Lobert; A Dewilde; P Wattré
Journal:  J Virol Methods       Date:  1996-10       Impact factor: 2.014

6.  Multicenter evaluating of a commercially available PCR assay for diagnosing enterovirus infection in a panel of cerebrospinal fluid specimens.

Authors:  B Lina; B Pozzetto; L Andreoletti; E Beguier; T Bourlet; E Dussaix; L Grangeot-Keros; B Gratacap-Cavallier; C Henquell; M C Legrand-Quillien; A Novillo; P Palmer; J Petitjean; K Sandres; P Dubreuil; H Fleury; F Freymuth; I Leparc-Goffart; D Hober; J Izopet; H Kopecka; Y Lazizi; H Lafeuille; P Lebon; A Roseto; E Marchadier; B Masquelier; B Picard; J Puel; J M Seigneurin; P Wattre; M Aymard
Journal:  J Clin Microbiol       Date:  1996-12       Impact factor: 5.948

7.  Aseptic meningitis in infants < 2 years of age: diagnosis and etiology.

Authors:  L E Berlin; M L Rorabaugh; F Heldrich; K Roberts; T Doran; J F Modlin
Journal:  J Infect Dis       Date:  1993-10       Impact factor: 5.226

8.  Comparison of cell cultures for rapid isolation of enteroviruses.

Authors:  T Chonmaitree; C Ford; C Sanders; H L Lucia
Journal:  J Clin Microbiol       Date:  1988-12       Impact factor: 5.948

Review 9.  Enteroviral infections of the central nervous system.

Authors:  H A Rotbart
Journal:  Clin Infect Dis       Date:  1995-04       Impact factor: 9.079

10.  Diagnosis of neonatal enterovirus infection by polymerase chain reaction.

Authors:  M J Abzug; M Loeffelholz; H A Rotbart
Journal:  J Pediatr       Date:  1995-03       Impact factor: 4.406

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  6 in total

1.  Multicenter quality assessment of PCR methods for detection of enteroviruses.

Authors:  P Muir; A Ras; P E Klapper; G M Cleator; K Korn; C Aepinus; A Fomsgaard; P Palmer; A Samuelsson; A Tenorio; B Weissbrich; A M van Loon
Journal:  J Clin Microbiol       Date:  1999-05       Impact factor: 5.948

2.  Diagnosis of enterovirus infection by genus-specific PCR and enzyme-linked immunosorbent assays.

Authors:  Maria E Craig; Peter Robertson; Neville J Howard; Martin Silink; William D Rawlinson
Journal:  J Clin Microbiol       Date:  2003-02       Impact factor: 5.948

3.  First epidemic of echovirus 16 meningitis in Cuba.

Authors:  L Sarmiento; P Mas; A Goyenechea; R Palomera; L Morier; V Capó; I Quintana; M Santin
Journal:  Emerg Infect Dis       Date:  2001 Sep-Oct       Impact factor: 6.883

4.  Typing of enteroviruses by use of microwell oligonucleotide arrays.

Authors:  P Susi; L Hattara; M Waris; T Luoma-Aho; H Siitari; T Hyypiä; P Saviranta
Journal:  J Clin Microbiol       Date:  2009-04-08       Impact factor: 5.948

5.  Development of a highly sensitive real-time one step RT-PCR combined complementary locked primer technology and conjugated minor groove binder probe.

Authors:  JiYoung Hong; Byunghak Kang; Ahyoun Kim; Seoyeon Hwang; Jinhee Ahn; Sunhwa Lee; Jonghyen Kim; Jae-Hak Park; Doo-Sung Cheon
Journal:  Virol J       Date:  2011-06-29       Impact factor: 4.099

Review 6.  Enterovirus and parechovirus infection in children: a brief overview.

Authors:  S C M de Crom; J W A Rossen; A M van Furth; C C Obihara
Journal:  Eur J Pediatr       Date:  2016-05-07       Impact factor: 3.183

  6 in total

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