Literature DB >> 9464421

An anaerobic, intrachamber incubator for growth of Methanosarcina spp. on methanol-containing solid media.

W W Metcalf1, J K Zhang, R S Wolfe.   

Abstract

To simplify the incubation of Methanosarcina spp. on solid agar medium, a two-port, manual, rectangular air lock was modified to serve as an anaerobic incubator. In one operation, it is possible to incubate 153 petri plates, the equivalent of 11 standard anaerobic jars, with plating efficiencies identical to those of traditional protocols.

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Year:  1998        PMID： 9464421      PMCID： PMC106116     

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  6 in total

1.  Transcriptional regulation in Archaea: in vivo demonstration of a repressor binding site in a methanogen.

Authors:  R Cohen-Kupiec; C Blank; J A Leigh
Journal:  Proc Natl Acad Sci U S A       Date:  1997-02-18       Impact factor: 11.205

Review 2.  Methanogens: reevaluation of a unique biological group.

Authors:  W E Balch; G E Fox; L J Magrum; C R Woese; R S Wolfe
Journal:  Microbiol Rev       Date:  1979-06

3.  A genetic system for Archaea of the genus Methanosarcina: liposome-mediated transformation and construction of shuttle vectors.

Authors:  W W Metcalf; J K Zhang; E Apolinario; K R Sowers; R S Wolfe
Journal:  Proc Natl Acad Sci U S A       Date:  1997-03-18       Impact factor: 11.205

4.  Integration of foreign DNA in an intergenic region of the archaeon Methanosarcina mazei without effect on transcription of adjacent genes.

Authors:  E Conway de Macario; M Guerrini; C B Dugan; A J Macario
Journal:  J Mol Biol       Date:  1996-09-13       Impact factor: 5.469

5.  Genetics in methanogens: transposon insertion mutagenesis of a Methanococcus maripaludis nifH gene.

Authors:  C E Blank; P S Kessler; J A Leigh
Journal:  J Bacteriol       Date:  1995-10       Impact factor: 3.490

6.  Molecular, genetic, and biochemical characterization of the serC gene of Methanosarcina barkeri Fusaro.

Authors:  W W Metcalf; J K Zhang; X Shi; R S Wolfe
Journal:  J Bacteriol       Date:  1996-10       Impact factor: 3.490
  6 in total
  25 in total

1.  The genome of M. acetivorans reveals extensive metabolic and physiological diversity.

Authors:  James E Galagan; Chad Nusbaum; Alice Roy; Matthew G Endrizzi; Pendexter Macdonald; Will FitzHugh; Sarah Calvo; Reinhard Engels; Serge Smirnov; Deven Atnoor; Adam Brown; Nicole Allen; Jerome Naylor; Nicole Stange-Thomann; Kurt DeArellano; Robin Johnson; Lauren Linton; Paul McEwan; Kevin McKernan; Jessica Talamas; Andrea Tirrell; Wenjuan Ye; Andrew Zimmer; Robert D Barber; Isaac Cann; David E Graham; David A Grahame; Adam M Guss; Reiner Hedderich; Cheryl Ingram-Smith; H Craig Kuettner; Joseph A Krzycki; John A Leigh; Weixi Li; Jinfeng Liu; Biswarup Mukhopadhyay; John N Reeve; Kerry Smith; Timothy A Springer; Lowell A Umayam; Owen White; Robert H White; Everly Conway de Macario; James G Ferry; Ken F Jarrell; Hua Jing; Alberto J L Macario; Ian Paulsen; Matthew Pritchett; Kevin R Sowers; Ronald V Swanson; Steven H Zinder; Eric Lander; William W Metcalf; Bruce Birren
Journal:  Genome Res       Date:  2002-04       Impact factor: 9.043

2.  Anaerobic life-a centennial view.

Authors:  R S Wolfe
Journal:  J Bacteriol       Date:  1999-06       Impact factor: 3.490

3.  Biochemical Characterization of the Methylmercaptopropionate:Cob(I)alamin Methyltransferase from Methanosarcina acetivorans.

Authors:  He Fu; Michelle N Goettge; William W Metcalf
Journal:  J Bacteriol       Date:  2019-05-22       Impact factor: 3.490

4.  New methods for tightly regulated gene expression and highly efficient chromosomal integration of cloned genes for Methanosarcina species.

Authors:  Adam M Guss; Michael Rother; Jun Kai Zhang; Gargi Kulkarni; William W Metcalf
Journal:  Archaea       Date:  2008-12       Impact factor: 3.273

5.  Cas9-mediated genome editing in the methanogenic archaeon Methanosarcina acetivorans.

Authors:  Dipti D Nayak; William W Metcalf
Journal:  Proc Natl Acad Sci U S A       Date:  2017-03-06       Impact factor: 11.205

6.  Differential regulation of the three methanol methyltransferase isozymes in Methanosarcina acetivorans C2A.

Authors:  Arpita Bose; Matthew A Pritchett; Michael Rother; William W Metcalf
Journal:  J Bacteriol       Date:  2006-10       Impact factor: 3.490

Review 7.  Stress genes and proteins in the archaea.

Authors:  A J Macario; M Lange; B K Ahring; E Conway de Macario
Journal:  Microbiol Mol Biol Rev       Date:  1999-12       Impact factor: 11.056

8.  Genetic, Genomic, and Transcriptomic Studies of Pyruvate Metabolism in Methanosarcina barkeri Fusaro.

Authors:  Madeline M López Muñoz; Peter Schönheit; William W Metcalf
Journal:  J Bacteriol       Date:  2015-09-08       Impact factor: 3.490

9.  Physiology and posttranscriptional regulation of methanol:coenzyme M methyltransferase isozymes in Methanosarcina acetivorans C2A.

Authors:  Rina B Opulencia; Arpita Bose; William W Metcalf
Journal:  J Bacteriol       Date:  2009-09-18       Impact factor: 3.490

10.  Development of a markerless genetic exchange method for Methanosarcina acetivorans C2A and its use in construction of new genetic tools for methanogenic archaea.

Authors:  Matthew A Pritchett; Jun Kai Zhang; William W Metcalf
Journal:  Appl Environ Microbiol       Date:  2004-03       Impact factor: 4.792
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