Literature DB >> 9453159

A reliable amplification technique for the characterization of genomic DNA sequences flanking insertion sequences.

G Prod'hom1, B Lagier, V Pelicic, A J Hance, B Gicquel, C Guilhot.   

Abstract

A simple and efficient ligation-mediated PCR (LMPCR) is described for amplifying DNA adjacent to known sequences. The method uses one primer specific for the known sequence and a second specific for a synthetic linker ligated to restricted genomic DNA. Perkin-Elmer AmpliTaq Gold polymerase is used to minimize non-specific primer annealing and amplification. This LMPCR method was successfully applied to isolate DNA sequences flanking mobile elements present in mycobacterial mutants generated by transposon mutagenesis.

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Year:  1998        PMID： 9453159     DOI： 10.1111/j.1574-6968.1998.tb12803.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  37 in total

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Journal:  J Bacteriol       Date:  2007-10-19       Impact factor: 3.490
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