Literature DB >> 9450549

Functional expression of pharaonis phoborhodopsin in Escherichia coli.

K Shimono1, M Iwamoto, M Sumi, N Kamo.   

Abstract

Pharaonis phoborhodopsin, the photoreceptor of the negative phototaxis of archaebacterial Natronobacterium pharaonis, was functionally expressed in the heterologous system of Escherichia coli. Flash-photolysis on a millisecond time scale indicated that the photochemical properties of ppR expressed in E. coli were the same as those of the native ppR in N. pharaonis. We concluded that the integral membrane protein ppR is correctly folded in vivo in the eubacterial E. coli membrane.

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Year:  1997        PMID： 9450549     DOI： 10.1016/s0014-5793(97)01487-7

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  29 in total

1.  The M intermediate of Pharaonis phoborhodopsin is photoactive.

Authors:  S P Balashov; M Sumi; N Kamo
Journal:  Biophys J       Date:  2000-06       Impact factor: 4.033

2.  Probing the proton channel and the retinal binding site of Natronobacterium pharaonis sensory rhodopsin II.

Authors:  Johann P Klare; Georg Schmies; Igor Chizhov; Kazumi Shimono; Naoki Kamo; Martin Engelhard
Journal:  Biophys J       Date:  2002-04       Impact factor: 4.033

3.  Reprogramming chaperone pathways to improve membrane protein expression in Escherichia coli.

Authors:  Brent L Nannenga; François Baneyx
Journal:  Protein Sci       Date:  2011-07-07       Impact factor: 6.725

4.  Tyr-199 and charged residues of pharaonis Phoborhodopsin are important for the interaction with its transducer.

Authors:  Yuki Sudo; Masayuki Iwamoto; Kazumi Shimono; Naoki Kamo
Journal:  Biophys J       Date:  2002-07       Impact factor: 4.033

5.  Electric-field dependent decays of two spectroscopically different M-states of photosensory rhodopsin II from Natronobacterium pharaonis.

Authors:  Laura Rivas; Silke Hippler-Mreyen; Martin Engelhard; Peter Hildebrandt
Journal:  Biophys J       Date:  2003-06       Impact factor: 4.033

6.  FTIR spectroscopy of the M photointermediate in pharaonis rhoborhodopsin.

Authors:  Yuji Furutani; Masayuki Iwamoto; Kazumi Shimono; Naoki Kamo; Hideki Kandori
Journal:  Biophys J       Date:  2002-12       Impact factor: 4.033

7.  The photochemical reaction cycle and photoinduced proton transfer of sensory rhodopsin II (Phoborhodopsin) from Halobacterium salinarum.

Authors:  Jun Tamogami; Takashi Kikukawa; Yoichi Ikeda; Ayaka Takemura; Makoto Demura; Naoki Kamo
Journal:  Biophys J       Date:  2010-04-07       Impact factor: 4.033

8.  Correlation of the O-intermediate rate with the pKa of Asp-75 in the dark, the counterion of the Schiff base of Pharaonis phoborhodopsin (sensory rhodopsin II).

Authors:  Masayuki Iwamoto; Yuki Sudo; Kazumi Shimono; Tsunehisa Araiso; Naoki Kamo
Journal:  Biophys J       Date:  2004-11-08       Impact factor: 4.033

9.  Role of Asp193 in chromophore-protein interaction of pharaonis phoborhodopsin (sensory rhodopsin II).

Authors:  Masayuki Iwamoto; Yuji Furutani; Yuki Sudo; Kazumi Shimono; Hideki Kandori; Naoki Kamo
Journal:  Biophys J       Date:  2002-08       Impact factor: 4.033

10.  Genetic cloning and functional expression in Escherichia coli of an archaerhodopsin gene from Halorubrum xinjiangense.

Authors:  Jie Feng; Hong-Can Liu; Jin-Fang Chu; Pei-Jin Zhou; Ji-An Tang; Shuang-Jiang Liu
Journal:  Extremophiles       Date:  2005-08-02       Impact factor: 2.395
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