Literature DB >> 9447911

Evaluation of a direct immunofluorescence cytospin assay for the detection of herpes simplex virus in clinical samples.

J Reina1, J Saurina, V Fernandez-Baca, M Munar, I Blanco.   

Abstract

A comparison between a direct immunofluorescence assay (DFA) and the shell-vial culture (SVC) was conducted to evaluate their efficacies according to the quality and origin of the sample and the type of herpes simplex (HSV) responsible for the infection. The SVC detected all 58 HSV-infected samples, while the DFA detected only 49 (84.5%) positive samples. The DFA detected HSV type 1 in 22 of 89 samples (24.7%) and HSV type 2 in 27 of 96 samples (28.1%). Compared with the SVC, the DFA had a sensitivity of 75.8% for HSV type 1 and 93.1% for HSV type 2. The sensitivity of the DFA depends on the quality of the sample. Thus, while DFA is recommendable as a screening method, the SVC remains the method of choice for obtaining the maximum diagnostic yield from the sample.

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Mesh：

Year:  1997        PMID： 9447911     DOI： 10.1007/bf01700419

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  9 in total

1.  Detection of herpes simplex virus in clinical specimens by cytospin-enhanced direct immunofluorescence.

Authors:  M L Landry; D Ferguson; J Wlochowski
Journal:  J Clin Microbiol       Date:  1997-01       Impact factor: 5.948

2.  Detection of herpes simplex virus in direct specimens by immunofluorescence assay using a monoclonal antibody.

Authors:  P Pouletty; J J Chomel; D Thouvenot; F Catalan; V Rabillon; J Kadouche
Journal:  J Clin Microbiol       Date:  1987-05       Impact factor: 5.948

3.  Comparison of the detection of herpes simplex virus in direct clinical specimens with herpes simplex virus-specific DNA probes and monoclonal antibodies.

Authors:  J C Fung; J Shanley; R C Tilton
Journal:  J Clin Microbiol       Date:  1985-11       Impact factor: 5.948

4.  Evaluation of two rapid methods for the detection of herpes simplex virus antigen in patient specimens.

Authors:  B E Hoffmann; D L Jungkind; G J Haller; R Sharrar; R A Baker; M Weisberg
Journal:  Ann Clin Lab Sci       Date:  1985 Sep-Oct       Impact factor: 1.256

5.  Identification and typing of herpes simplex viruses with monoclonal antibodies.

Authors:  N Balachandran; B Frame; M Chernesky; E Kraiselburd; Y Kouri; D Garcia; C Lavery; W E Rawls
Journal:  J Clin Microbiol       Date:  1982-07       Impact factor: 5.948

6.  Comparison of direct immunofluorescence and direct immunoperoxidase procedures for detection of herpes simplex virus antigen in lesion specimens.

Authors:  N J Schmidt; J Dennis; V Devlin; D Gallo; J Mills
Journal:  J Clin Microbiol       Date:  1983-08       Impact factor: 5.948

7.  Comparison of different immunostaining techniques and monoclonal antibodies to the lower matrix phosphoprotein (pp65) for optimal quantitation of human cytomegalovirus antigenemia.

Authors:  G Gerna; M G Revello; E Percivalle; F Morini
Journal:  J Clin Microbiol       Date:  1992-05       Impact factor: 5.948

8.  Monoclonal antibodies to herpes simplex viruses: use in antigenic typing and rapid diagnosis.

Authors:  L C Goldstein; L Corey; J K McDougall; E Tolentino; R C Nowinski
Journal:  J Infect Dis       Date:  1983-05       Impact factor: 5.226

9.  Comparison of viral isolation, direct immunofluorescence, and indirect immunoperoxidase techniques for detection of genital herpes simplex virus infection.

Authors:  R C Moseley; L Corey; D Benjamin; C Winter; M L Remington
Journal:  J Clin Microbiol       Date:  1981-05       Impact factor: 5.948
  9 in total
  1 in total

Review 1.  Light microscopy, culture, molecular, and serologic methods for detection of herpes simplex virus.

Authors:  Neil W Anderson; Blake W Buchan; Nathan A Ledeboer
Journal:  J Clin Microbiol       Date:  2013-10-16       Impact factor: 5.948
  1 in total

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