Literature DB >> 3034970

Detection of herpes simplex virus in direct specimens by immunofluorescence assay using a monoclonal antibody.

P Pouletty, J J Chomel, D Thouvenot, F Catalan, V Rabillon, J Kadouche.   

Abstract

A monoclonal antibody (MAb), designated CHA 437, was developed against herpes simplex virus (HSV). This MAb (isotype, immunoglobulin G2b K) reacted with HSV type 1 and HSV type 2. It showed no cross-reactivity with varicella-zoster virus, cytomegalovirus, or Epstein-Barr virus. Direct detection of HSV antigen in clinical specimens using indirect immunofluorescence with this MAb was compared with tissue culture isolation. For the 682 specimens tested, the direct specimen test gave a sensitivity of 84.6% and a specificity of 95.7%.

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Year:  1987        PMID： 3034970      PMCID： PMC266133          DOI： 10.1128/jcm.25.5.958-959.1987

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

1.  Comparison of the detection of herpes simplex virus in direct clinical specimens with herpes simplex virus-specific DNA probes and monoclonal antibodies.

Authors:  J C Fung; J Shanley; R C Tilton
Journal:  J Clin Microbiol       Date:  1985-11       Impact factor: 5.948

2.  Evaluation of two rapid methods for the detection of herpes simplex virus antigen in patient specimens.

Authors:  B E Hoffmann; D L Jungkind; G J Haller; R Sharrar; R A Baker; M Weisberg
Journal:  Ann Clin Lab Sci       Date:  1985 Sep-Oct       Impact factor: 1.256

3.  Serological analysis of herpes simplex virus types 1 and 2 with monoclonal antibodies.

Authors:  L Pereira; D V Dondero; D Gallo; V Devlin; J D Woodie
Journal:  Infect Immun       Date:  1982-01       Impact factor: 3.441

4.  Identification and typing of herpes simplex viruses with monoclonal antibodies.

Authors:  N Balachandran; B Frame; M Chernesky; E Kraiselburd; Y Kouri; D Garcia; C Lavery; W E Rawls
Journal:  J Clin Microbiol       Date:  1982-07       Impact factor: 5.948

5.  Diagnosis of Chlamydia trachomatis infections by direct immunofluorescence staining of genital secretions. A multicenter trial.

Authors:  W E Stamm; H R Harrison; E R Alexander; L D Cles; M R Spence; T C Quinn
Journal:  Ann Intern Med       Date:  1984-11       Impact factor: 25.391

6.  Respiratory syncytial virus detection by immunofluorescence in nasal secretions with monoclonal antibodies against selected surface and internal proteins.

Authors:  H W Kim; R G Wyatt; B F Fernie; C D Brandt; J O Arrobio; B C Jeffries; R H Parrott
Journal:  J Clin Microbiol       Date:  1983-12       Impact factor: 5.948

7.  Monoclonal antibodies to herpes simplex viruses: use in antigenic typing and rapid diagnosis.

Authors:  L C Goldstein; L Corey; J K McDougall; E Tolentino; R C Nowinski
Journal:  J Infect Dis       Date:  1983-05       Impact factor: 5.226
  7 in total
  6 in total

1.  Evaluation of a direct immunofluorescence cytospin assay for the detection of herpes simplex virus in clinical samples.

Authors:  J Reina; J Saurina; V Fernandez-Baca; M Munar; I Blanco
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1997-11       Impact factor: 3.267

2.  Detection of herpes simplex virus in clinical specimens by cytospin-enhanced direct immunofluorescence.

Authors:  M L Landry; D Ferguson; J Wlochowski
Journal:  J Clin Microbiol       Date:  1997-01       Impact factor: 5.948

3.  Diagnosis of herpes simplex virus infection in a clinical setting by a direct antigen detection enzyme immunoassay kit.

Authors:  A Dascal; J Chan-Thim; M Morahan; J Portnoy; J Mendelson
Journal:  J Clin Microbiol       Date:  1989-04       Impact factor: 5.948

4.  Optimization of a rapid test by using fluorescein-conjugated monoclonal antibodies for detection of Chlamydia trachomatis in clinical specimens.

Authors:  P Pouletty; J Martin; F Catalan; M Garcia-Gonzalez; I Morellet; S Bettinger; J Kadouche
Journal:  J Clin Microbiol       Date:  1988-02       Impact factor: 5.948

5.  Detection and direct typing of herpes simplex virus by polymerase chain reaction.

Authors:  H Kimura; M Shibata; K Kuzushima; K Nishikawa; Y Nishiyama; T Morishima
Journal:  Med Microbiol Immunol       Date:  1990       Impact factor: 3.402

6.  Pregnancy and sexually transmitted viral infections.

Authors:  P Singhal; S Naswa; Y S Marfatia
Journal:  Indian J Sex Transm Dis AIDS       Date:  2009-07
  6 in total

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