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Literature DB >> 9445081

Construction and characterization of a hybrid mouse mammary tumor virus/murine leukemia virus-based retroviral vector.

R M Saller¹, F Oztürk, B Salmons, W H Günzburg.

Abstract

Mouse mammary tumor virus (MMTV)-based vectors are characterized by low titers. In an effort to transfer MMTV-specific regulation of gene expression to a more efficient murine leukemia virus (MLV) vector, we have replaced the complete 3' U3 region of MLV with the complete U3 region of MMTV. Virus titers were not significantly affected by this modification, there was no impairment of reverse transcription and integration, and after infection of cells, the MMTV promoter is duplicated and translocated to the 5' long terminal repeat, resulting in glucocorticoid-regulatable RNA expression.

Entities: Disease Species

Mesh：

Year: 1998 PMID： 9445081 PMCID： PMC124659

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

23 in total

Review 1. Factors controlling the expression of mouse mammary tumour virus.

Authors: W H Günzburg; B Salmons
Journal: Biochem J Date: 1992-05-01 Impact factor: 3.857

Review 2. Genetics of retroviral integration.

Authors: S P Goff
Journal: Annu Rev Genet Date: 1992 Impact factor: 16.830

3. Murine leukemia virus-based Tat-inducible long terminal repeat replacement vectors: a new system for anti-human immunodeficiency virus gene therapy.

Authors: P M Cannon; N Kim; S M Kingsman; A J Kingsman
Journal: J Virol Date: 1996-11 Impact factor: 5.103

4. Generation of glucocorticoid-responsive Moloney murine leukemia virus by insertion of regulatory sequences from murine mammary tumor virus into the long terminal repeat.

Authors: J Overhauser; H Fan
Journal: J Virol Date: 1985-04 Impact factor: 5.103

5. Construction of a clonable, infectious, and tumorigenic mouse mammary tumor virus provirus and a derivative genetic vector.

Authors: G M Shackleford; H E Varmus
Journal: Proc Natl Acad Sci U S A Date: 1988-12 Impact factor: 11.205

6. Negative regulation in correct tissue-specific expression of mouse mammary tumor virus in transgenic mice.

Authors: S R Ross; C L Hsu; Y Choi; E Mok; J P Dudley
Journal: Mol Cell Biol Date: 1990-11 Impact factor: 4.272

7. Improved gene expression upon transfer of the adenosine deaminase minigene outside the transcriptional unit of a retroviral vector.

Authors: P A Hantzopoulos; B A Sullenger; G Ungers; E Gilboa
Journal: Proc Natl Acad Sci U S A Date: 1989-05 Impact factor: 11.205

8. A mouse mammary tumor virus mammary gland enhancer confers tissue-specific but not lactation-dependent expression in transgenic mice.

Authors: E Mok; T V Golovkina; S R Ross
Journal: J Virol Date: 1992-12 Impact factor: 5.103

9. A mammary cell-specific enhancer in mouse mammary tumor virus DNA is composed of multiple regulatory elements including binding sites for CTF/NFI and a novel transcription factor, mammary cell-activating factor.

Authors: S Mink; E Härtig; P Jennewein; W Doppler; A C Cato
Journal: Mol Cell Biol Date: 1992-11 Impact factor: 4.272