Literature DB >> 9438261

Direct sequencing of unpurified PCR-amplified DNA by semi-exponential cycle sequencing (SECS).

G Sarkar1, M E Bolander.   

Abstract

A simple technique for direct sequencing of PCR-amplified templates without purification of the PCR reaction product is presented. This method does not require an additional synthesis step after template amplification, and can generate sequence information form as little as 0.1 fmol of unpurified template.

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Year:  1997        PMID： 9438261     DOI： 10.1007/BF02760780

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  11 in total

1.  Sequencing products of the polymerase chain reaction directly, without purification.

Authors:  S J Meltzer; S M Mane; P K Wood; L Johnson; S W Needleman
Journal:  Biotechniques       Date:  1990-02       Impact factor: 1.993

2.  Rapid one-step characterization of recombinant vectors by direct analysis of transformed Escherichia coli colonies.

Authors:  G S Sandhu; J W Precup; B C Kline
Journal:  Biotechniques       Date:  1989 Jul-Aug       Impact factor: 1.993

3.  Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.

Authors:  K B Mullis; F A Faloona
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

4.  Semi exponential cycle sequencing.

Authors:  G Sarkar; M E Bolander
Journal:  Nucleic Acids Res       Date:  1995-04-11       Impact factor: 16.971

5.  Restriction-site PCR: a direct method of unknown sequence retrieval adjacent to a known locus by using universal primers.

Authors:  G Sarkar; R T Turner; M E Bolander
Journal:  PCR Methods Appl       Date:  1993-05

6.  Rapid sequencing of unpurified PCR products by thermal asymmetric PCR cycle sequencing using unlabeled sequencing primers.

Authors:  Y G Liu; N Mitsukawa; R F Whittier
Journal:  Nucleic Acids Res       Date:  1993-07-11       Impact factor: 16.971

7.  A rapid polymerase-chain-reaction-directed sequencing strategy using a thermostable DNA polymerase from Thermus flavus.

Authors:  V B Rao; N B Saunders
Journal:  Gene       Date:  1992-04-01       Impact factor: 3.688

8.  A system for shotgun DNA sequencing.

Authors:  J Messing; R Crea; P H Seeburg
Journal:  Nucleic Acids Res       Date:  1981-01-24       Impact factor: 16.971

9.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA.

Authors:  M A Innis; K B Myambo; D H Gelfand; M A Brow
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205
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  2 in total

1.  Gene digging. A method for obtaining species-specific sequence based on conserved segments of nucleotides in open reading frames.

Authors:  A Sanyal; S W O'Driscoll; J S Fitzsimmons; M E Bolander; G Sarkar
Journal:  Mol Biotechnol       Date:  1998-12       Impact factor: 2.695

2.  Natural transformation of Campylobacter jejuni requires components of a type II secretion system.

Authors:  Rebecca S Wiesner; David R Hendrixson; Victor J DiRita
Journal:  J Bacteriol       Date:  2003-09       Impact factor: 3.490
  2 in total

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