Literature DB >> 9421533

Microinjection of cre recombinase RNA induces site-specific recombination of a transgene in mouse oocytes.

T de Wit1, D Drabek, F Grosveld.   

Abstract

We have developed a strategy for producing single copy transgenic mouse lines using Cre-loxP site specific recombination. The method is based on transient expression of the recombinase after injection of in vitro transcribed mRNA into the cytoplasm of fertilised eggs containing multiple copies of the transgene. The success rate of the recombination event is 100% (15 out of 15).

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Year:  1998        PMID： 9421533      PMCID： PMC147291          DOI： 10.1093/nar/26.2.676

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  18 in total

1.  Internal initiation of translation mediated by the 5' leader of a cellular mRNA.

Authors:  D G Macejak; P Sarnow
Journal:  Nature       Date:  1991-09-05       Impact factor: 49.962

2.  Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase.

Authors:  B Sauer; N Henderson
Journal:  New Biol       Date:  1990-05

3.  Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1.

Authors:  B Sauer; N Henderson
Journal:  Proc Natl Acad Sci U S A       Date:  1988-07       Impact factor: 11.205

4.  A segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation.

Authors:  S K Jang; H G Kräusslich; M J Nicklin; G M Duke; A C Palmenberg; E Wimmer
Journal:  J Virol       Date:  1988-08       Impact factor: 5.103

5.  Dicistronic targeting constructs: reporters and modifiers of mammalian gene expression.

Authors:  P Mountford; B Zevnik; A Düwel; J Nichols; M Li; C Dani; M Robertson; I Chambers; A Smith
Journal:  Proc Natl Acad Sci U S A       Date:  1994-05-10       Impact factor: 11.205

6.  Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.

Authors:  D A Melton; P A Krieg; M R Rebagliati; T Maniatis; K Zinn; M R Green
Journal:  Nucleic Acids Res       Date:  1984-09-25       Impact factor: 16.971

7.  Synthesis of functional mRNA in mammalian cells by bacteriophage T3 RNA polymerase.

Authors:  Y Zhou; T J Giordano; R K Durbin; W T McAllister
Journal:  Mol Cell Biol       Date:  1990-09       Impact factor: 4.272

8.  Cap-independent translation of mRNA conferred by encephalomyocarditis virus 5' sequence improves the performance of the vaccinia virus/bacteriophage T7 hybrid expression system.

Authors:  O Elroy-Stein; T R Fuerst; B Moss
Journal:  Proc Natl Acad Sci U S A       Date:  1989-08       Impact factor: 11.205

9.  Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA.

Authors:  J Pelletier; N Sonenberg
Journal:  Nature       Date:  1988-07-28       Impact factor: 49.962

10.  Genomic targeting with purified Cre recombinase.

Authors:  W Baubonis; B Sauer
Journal:  Nucleic Acids Res       Date:  1993-05-11       Impact factor: 16.971
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  10 in total

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2.  Generation of a Slc39a8 hypomorph mouse: markedly decreased ZIP8 Zn²⁺/(HCO₃⁻)₂ transporter expression.

Authors:  Bin Wang; Lei He; Hongbin Dong; Timothy P Dalton; Daniel W Nebert
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Review 3.  Exogenous enzymes upgrade transgenesis and genetic engineering of farm animals.

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Journal:  Cell Mol Life Sci       Date:  2015-02-01       Impact factor: 9.261

4.  Specificity protein 2 (Sp2) is essential for mouse development and autonomous proliferation of mouse embryonic fibroblasts.

Authors:  Frank Baur; Kerstin Nau; Dennis Sadic; Lena Allweiss; Hans-Peter Elsässer; Nynke Gillemans; Ton de Wit; Imme Krüger; Marion Vollmer; Sjaak Philipsen; Guntram Suske
Journal:  PLoS One       Date:  2010-03-08       Impact factor: 3.240

5.  Mesoporous silica nanoparticle-mediated intracellular cre protein delivery for maize genome editing via loxP site excision.

Authors:  Susana Martin-Ortigosa; David J Peterson; Justin S Valenstein; Victor S-Y Lin; Brian G Trewyn; L Alexander Lyznik; Kan Wang
Journal:  Plant Physiol       Date:  2013-12-27       Impact factor: 8.340

6.  Stella-Cre mice are highly efficient Cre deleters.

Authors:  Hui Liu; Wei Wang; Su-Kit Chew; Song-Choon Lee; Juan Li; George S Vassiliou; Tony Green; P Andrew Futreal; Allan Bradley; Shujun Zhang; Pentao Liu
Journal:  Genesis       Date:  2011-07-22       Impact factor: 2.487

7.  Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.

Authors:  Masato Ohtsuka; Sanae Ogiwara; Hiromi Miura; Akiko Mizutani; Takayuki Warita; Masahiro Sato; Kenji Imai; Katsuto Hozumi; Takehito Sato; Masafumi Tanaka; Minoru Kimura; Hidetoshi Inoko
Journal:  Nucleic Acids Res       Date:  2010-09-29       Impact factor: 16.971

Review 8.  Recombinase technology: applications and possibilities.

Authors:  Yueju Wang; Yuan-Yeu Yau; Donna Perkins-Balding; James G Thomson
Journal:  Plant Cell Rep       Date:  2010-10-24       Impact factor: 4.570

9.  Phage integrases for the construction and manipulation of transgenic mammals.

Authors:  Roger P Hollis; Stephanie M Stoll; Christopher R Sclimenti; Jennifer Lin; Yanru Chen-Tsai; Michele P Calos
Journal:  Reprod Biol Endocrinol       Date:  2003-11-07       Impact factor: 5.211

10.  Rapid conversion of EUCOMM/KOMP-CSD alleles in mouse embryos using a cell-permeable Cre recombinase.

Authors:  Edward Ryder; Brendan Doe; Diane Gleeson; Richard Houghton; Priya Dalvi; Evelyn Grau; Bishoy Habib; Evelina Miklejewska; Stuart Newman; Debarati Sethi; Caroline Sinclair; Sapna Vyas; Hannah Wardle-Jones; Joanna Bottomley; James Bussell; Antonella Galli; Jennifer Salisbury; Ramiro Ramirez-Solis
Journal:  Transgenic Res       Date:  2013-11-07       Impact factor: 2.788
  10 in total

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