BACKGROUND: Myeloid cell development is controlled by tissue-specific transcription factors. Human myeloid zinc finger protein (MZF-1) is a putative transcription factor containing 13 zinc fingers, and has been suggested that it regulates the development of neutrophilic granulocytes. RESULTS: Here, we have isolated the murine and human cDNAs which encode a novel form of MZF protein (MZF-2). Murine and human MZF-2 proteins consisted of 814 and 775 amino acids, respectively, and have identity of 75.3% between them. The C-terminal half of human MZF-2, carrying the zinc finger domains, was completely identical with that of human MZF-1, whereas the N-terminal half of MZF-2 was different from the corresponding region of human MZF-1, and was coded by distinct exons. MZF-2 mRNA was expressed in myeloid cells, particularly in the cells committed to the neutrophilic lineage, and down-regulated by G-CSF. CONCLUSIONS: MZF-1 and MZF-2 mRNAs seem to be produced by the alternative use of two different transcription initiation sites. The distinct N-terminal half of MZF-2 carries two characteristic domains, a leucine-rich domain called LeR and an acidic domain, which suggests a unique function of MZF-2 in neutrophil development.
BACKGROUND: Myeloid cell development is controlled by tissue-specific transcription factors. Human myeloid zinc finger protein (MZF-1) is a putative transcription factor containing 13 zinc fingers, and has been suggested that it regulates the development of neutrophilic granulocytes. RESULTS: Here, we have isolated the murine and human cDNAs which encode a novel form of MZF protein (MZF-2). Murine and human MZF-2 proteins consisted of 814 and 775 amino acids, respectively, and have identity of 75.3% between them. The C-terminal half of human MZF-2, carrying the zinc finger domains, was completely identical with that of humanMZF-1, whereas the N-terminal half of MZF-2 was different from the corresponding region of humanMZF-1, and was coded by distinct exons. MZF-2 mRNA was expressed in myeloid cells, particularly in the cells committed to the neutrophilic lineage, and down-regulated by G-CSF. CONCLUSIONS:MZF-1 and MZF-2 mRNAs seem to be produced by the alternative use of two different transcription initiation sites. The distinct N-terminal half of MZF-2 carries two characteristic domains, a leucine-rich domain called LeR and an acidic domain, which suggests a unique function of MZF-2 in neutrophil development.
Authors: M Gaboli; P A Kotsi; C Gurrieri; G Cattoretti; S Ronchetti; C Cordon-Cardo; H E Broxmeyer; R Hromas; P P Pandolfi Journal: Genes Dev Date: 2001-07-01 Impact factor: 11.361
Authors: Dan Wu; Hua Tan; Weijun Su; Dongmei Cheng; Guanwen Wang; Juan Wang; Ding A Ma; George M Dong; Peiqing Sun Journal: Oncogene Date: 2021-11-12 Impact factor: 9.867