Literature DB >> 9406189

The sacB gene cannot be used as a counter-selectable marker in Pasteurella multocida.

B H Jost1, P Homchampa, R A Strugnell, B Adler.   

Abstract

The use of the Bacillus subtilis sacB gene as a counter-selectable marker was assessed in serogroup A and B strains of Pasteurella multocida. Expression ofsacB failed to render any of the strains sensitive to sucrose, indicating that the sacB gene can not be used as a positive selection system in P. multocida.

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Year:  1997        PMID： 9406189     DOI： 10.1007/bf02752263

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  8 in total

1.  Expression of the Bacillus subtilis sacB gene leads to sucrose sensitivity in the gram-positive bacterium Corynebacterium glutamicum but not in Streptomyces lividans.

Authors:  W Jäger; A Schäfer; A Pühler; G Labes; W Wohlleben
Journal:  J Bacteriol       Date:  1992-08       Impact factor: 3.490

2.  Allelic exchange in Pseudomonas aeruginosa using novel ColE1-type vectors and a family of cassettes containing a portable oriT and the counter-selectable Bacillus subtilis sacB marker.

Authors:  H P Schweizer
Journal:  Mol Microbiol       Date:  1992-05       Impact factor: 3.501

3.  Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC101 replicon.

Authors:  I C Blomfield; V Vaughn; R F Rest; B I Eisenstein
Journal:  Mol Microbiol       Date:  1991-06       Impact factor: 3.501

4.  An nptI-sacB-sacR cartridge for constructing directed, unmarked mutations in gram-negative bacteria by marker exchange-eviction mutagenesis.

Authors:  J L Ried; A Collmer
Journal:  Gene       Date:  1987       Impact factor: 3.688

5.  Cross protective immunity conferred by a marker-free aroA mutant of Pasteurella multocida.

Authors:  P Homchampa; R A Strugnell; B Adler
Journal:  Vaccine       Date:  1997-02       Impact factor: 3.641

6.  Molecular analysis of the aroA gene of Pasteurella multocida and vaccine potential of a constructed aroA mutant.

Authors:  P Homchampa; R A Strugnell; B Adler
Journal:  Mol Microbiol       Date:  1992-12       Impact factor: 3.501

7.  Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria.

Authors:  P Gay; D Le Coq; M Steinmetz; T Berkelman; C I Kado
Journal:  J Bacteriol       Date:  1985-11       Impact factor: 3.490

8.  Molecular, genetic, and topological characterization of O-antigen chain length regulation in Shigella flexneri.

Authors:  R Morona; L van den Bosch; P A Manning
Journal:  J Bacteriol       Date:  1995-02       Impact factor: 3.490
  8 in total
  2 in total

1.  Protective immunity conferred by attenuated aroA derivatives of Pasteurella multocida B:2 strains in a mouse model of hemorrhagic septicemia.

Authors:  Mohammad Tabatabaei; Zhiqi Liu; Anna Finucane; Roger Parton; John Coote
Journal:  Infect Immun       Date:  2002-07       Impact factor: 3.441

2.  Genome modifications and cloning using a conjugally transferable recombineering system.

Authors:  Mohammad J Hossain; Charles M Thurlow; Dawei Sun; Shamima Nasrin; Mark R Liles
Journal:  Biotechnol Rep (Amst)       Date:  2015-08-28
  2 in total

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