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Literature DB >> 9406185

Generation of large insert yeast artificial chromosome libraries.

Abstract

The development of YAC cloning technology has directly enhanced the relationship among genetic, physical, and functional mapping of genomes. Because of their large size, YACs have enabled the rapid construction of physical maps by ordered clone mapping and contig building, and they complement other molecular approaches for mapping complex genomes. Large insert libraries are constructed by size fractionating large DNA embedded in agarose and protecting DNA from degradation with polyamines.

Entities: Chemical Species

Mesh：

Year: 1997 PMID： 9406185 DOI： 10.1007/BF02752259

Source DB: PubMed Journal: Mol Biotechnol ISSN： 1073-6085 Impact factor: 2.695

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References

23 in total

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