Literature DB >> 1302006

Construction of a mouse yeast artificial chromosome library in a recombination-deficient strain of yeast.

F L Chartier1, J T Keer, M J Sutcliffe, D A Henriques, P Mileham, S D Brown.   

Abstract

We have constructed a new generation yeast artificial chromosome (YAC) library from female C57BL/10 mice in a recombination-deficient strain of Saccharomyces cerevisiae carrying a mutation in the RAD52 gene. The YAC library contains 41,568 clones with an average insert size of 240 kilobases, representing a greater than threefold coverage of the mouse genome. Currently, the library can be screened by polymerase chain reaction and we have isolated positive clones at a number of loci in the mouse genome. This rad52 library should enable a long-term assessment of the effect of one of the yeast recombination pathway genes on both, genome-wide YAC clone stability and the frequency of chimaeric clones.

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Year:  1992        PMID： 1302006     DOI： 10.1038/ng0592-132

Source DB:  PubMed          Journal:  Nat Genet        ISSN： 1061-4036            Impact factor:   38.330


  26 in total

1.  Cloning and characterization of the 5'-upstream sequence governing the cell cycle-dependent transcription of mouse DNA polymerase alpha 68 kDa subunit gene.

Authors:  N S Nishikawa; M Izumi; H Uchida; M Yokoi; H Miyazawa; F Hanaoka
Journal:  Nucleic Acids Res       Date:  2000-04-01       Impact factor: 16.971

2.  A comprehensive large-insert yeast artificial chromosome library for physical mapping of the mouse genome.

Authors:  M L Haldi; C Strickland; P Lim; V VanBerkel; X Chen; D Noya; J R Korenberg; Z Husain; J Miller; E S Lander
Journal:  Mamm Genome       Date:  1996-10       Impact factor: 2.957

3.  Vector-hexamer PCR isolation of all insert ends from a YAC contig of the mouse Igh locus.

Authors:  C D Herring; C Chevillard; S L Johnston; P J Wettstein; R Riblet
Journal:  Genome Res       Date:  1998-06       Impact factor: 9.043

4.  Generation of large insert yeast artificial chromosome libraries.

Authors:  Z Larin; A P Monaco; H Lehrach
Journal:  Mol Biotechnol       Date:  1997-10       Impact factor: 2.695

5.  Rapid cloning of mouse DNA as yeast artificial chromosomes by transformation-associated recombination (TAR).

Authors:  M R Cancilla; J Graves; L E Matesic; R H Reeves; K M Tainton; K H Choo; M A Resnick; V L Larionov; N Y Kouprina
Journal:  Mamm Genome       Date:  1998-02       Impact factor: 2.957

6.  YAC clone contigs covering 5 Mb of a repeat sequence island on the mouse X chromosome.

Authors:  P Mileham; S D Brown
Journal:  Mamm Genome       Date:  1996-04       Impact factor: 2.957

Review 7.  Physical mapping of the rice genome with BACs.

Authors:  H B Zhang; R A Wing
Journal:  Plant Mol Biol       Date:  1997-09       Impact factor: 4.076

8.  Mutation of the Sry-related Sox10 gene in Dominant megacolon, a mouse model for human Hirschsprung disease.

Authors:  B Herbarth; V Pingault; N Bondurand; K Kuhlbrodt; I Hermans-Borgmeyer; A Puliti; N Lemort; M Goossens; M Wegner
Journal:  Proc Natl Acad Sci U S A       Date:  1998-04-28       Impact factor: 11.205

9.  Mutations in a NIMA-related kinase gene, Nek1, cause pleiotropic effects including a progressive polycystic kidney disease in mice.

Authors:  P Upadhya; E H Birkenmeier; C S Birkenmeier; J E Barker
Journal:  Proc Natl Acad Sci U S A       Date:  2000-01-04       Impact factor: 11.205

Review 10.  Mapping the mouse genome: current status and future prospects.

Authors:  W F Dietrich; N G Copeland; D J Gilbert; J C Miller; N A Jenkins; E S Lander
Journal:  Proc Natl Acad Sci U S A       Date:  1995-11-21       Impact factor: 11.205
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