Interspecies comparison of rat and hamster alveolar macrophage antioxidative and oxidative capacity.

Generation of oxidants has been implicated in lung injury and disease caused by a variety of inhaled agents such as ozone, particles, and mineral fibers. Antioxidants in the pulmonary system presumably provide the initial defense against such oxidants. We designed the present study to assess the oxidative and antioxidative capacity of alveolar macrophages (AM) from rats and hamsters. These two laboratory animal species commonly used in biomedical research are well known for their disparate response to pulmonary irritants/toxicants. AM from CD rats and Syrian golden hamsters were obtained by bronchoalveolar lavage. We assessed AM antioxidant levels by measuring the catalase and superoxide dismutase (SOD) activity and the intracellular concentrations of total glutathione, ascorbic acid, and alpha-tocopherol. We determined the AM oxidative capacity by assessing the ability of AM to oxidize extracellular glutathione (GSH) and to release superoxide anions. There were no significant differences in the intracellular antioxidant levels, except for catalase activity that was significantly (p < 0.05) higher in hamster AM than in rat AM. However, AM oxidative capacity was markedly different between the two species studied. The amount of spontaneous and phorbol myristate acetate (PMA)-induced GSH oxidation was about 5-fold higher in rat AM than in hamster AM, whereas the PMA-induced superoxide anion release did not differ significantly between the two rodents. In summary, our data suggest that species variation exists between the oxidative capacity of rat and that of hamster AM. Whereas the oxidative capacity of hamster AM appears to be based mainly on the formation of reactive oxygen species, it is suggested that rat AM possess an additional oxidative system.