Literature DB >> 9397517

Two-photon excitation of ethidium bromide labeled DNA.

H Malak1, F N Castellano, I Gryczynski, J R Lakowicz.   

Abstract

We examined the steady state and time-resolved emission of DNA stained with ethidium bromide (EB) when excited with 90 fs pulses from a mode-locked titanium sapphire laser. Over the wavelength range from 840 to 880 nm EB-DNA was found to display two-photon excitation, with a cross-section near 7 x 10(-50) cm4s/photon. Frequency-domain intensity decay measurements revealed similar multi-exponential intensity decays for one- and two-photon excitation. Time-resolved anisotropy decay measurements revealed similar correlation times, but different amplitudes as has been observed previously for two- versus one-photon excitation. These results indicate that two-photon excitation of EB-DNA can be accomplished with the fundamental output of a Ti:sapphire laser without obvious heating or perturbation of the DNA.

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Year:  1997        PMID： 9397517     DOI： 10.1016/s0301-4622(97)00017-3

Source DB:  PubMed          Journal:  Biophys Chem        ISSN： 0301-4622            Impact factor:   2.352


  8 in total

1.  One- and two-photon excited fluorescence lifetimes and anisotropy decays of green fluorescent proteins.

Authors:  A Volkmer; V Subramaniam; D J Birch; T M Jovin
Journal:  Biophys J       Date:  2000-03       Impact factor: 4.033

2.  A series of carbazole cationic compounds with large two-photon absorption cross sections for imaging mitochondria in living cells with two-photon fluorescence microscopy.

Authors:  Xin Liu; Yuming Sun; Yuanhong Zhang; Ning Zhao; Hongshi Zhao; Guancong Wang; Xiaoqiang Yu; Hong Liu
Journal:  J Fluoresc       Date:  2010-10-16       Impact factor: 2.217

3.  Toward the measurement of multiple fluorescence lifetimes in flow cytometry: maximizing multi-harmonic content from cells and microspheres.

Authors:  Patrick Jenkins; Mark A Naivar; Jessica P Houston
Journal:  J Biophotonics       Date:  2015-02-26       Impact factor: 3.207

4.  Directional two-photon induced surface plasmon-coupled emission.

Authors:  Ignacy Gryczynski; Joanna Malicka; Joseph R Lakowicz; Ewa M Goldys; Nils Calander; Zygmunt Gryczynski
Journal:  Thin Solid Films       Date:  2005-07-11       Impact factor: 2.183

5.  One- and two-photon induced fluorescence of Pacific Blue-labeled human serum albumin deposited on different core size silver colloids.

Authors:  Joanna Lukomska; Ignacy Gryczynski; Joanna Malicka; Slawomir Makowiec; Joseph R Lakowicz; Zygmunt Gryczynski
Journal:  Biopolymers       Date:  2006-03       Impact factor: 2.505

6.  Two-photon induced fluorescence of Cy5-DNA in buffer solution and on silver island films.

Authors:  Joanna Lukomska; Ignacy Gryczynski; Joanna Malicka; Slawomir Makowiec; Joseph R Lakowicz; Zygmunt Gryczynski
Journal:  Biochem Biophys Res Commun       Date:  2005-03-04       Impact factor: 3.575

7.  Rotation of the lever arm of Myosin in contracting skeletal muscle fiber measured by two-photon anisotropy.

Authors:  J Borejdo; A Shepard; I Akopova; W Grudzinski; J Malicka
Journal:  Biophys J       Date:  2004-09-17       Impact factor: 4.033

8.  Biomolecule labeling and imaging with a new fluorenyl two-photon fluorescent probe.

Authors:  Alma R Morales; Ciceron O Yanez; Katherine J Schafer-Hales; Adam I Marcus; Kevin D Belfield
Journal:  Bioconjug Chem       Date:  2009-10-02       Impact factor: 4.774
  8 in total

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