| Literature DB >> 15670753 |
Joanna Lukomska1, Ignacy Gryczynski, Joanna Malicka, Slawomir Makowiec, Joseph R Lakowicz, Zygmunt Gryczynski.
Abstract
We report the observation of a strong two-photon induced fluorescence emission of Cy5-DNA within the tunable range of a Ti:Sapphire laser. The estimated two-photon cross-section for Cy5-DNA of 400GM is about 3.5-fold higher than it was reported for rhodamine B. The fundamental anisotropies of Cy5-DNA are close to the theoretical limits of 2/5 and 4/7 for one- and two-photon excitation, respectively. We also observed an enhanced two-photon induced fluorescence (TPIF) of Cy5-DNA deposited on silver island films (SIFs). In the presence of SIFs, the TPIF is about 100-fold brighter. The brightness increase of Cy5-DNA TPIF near SIFs is mostly due to enhanced local field.Entities:
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Year: 2005 PMID: 15670753 PMCID: PMC6844251 DOI: 10.1016/j.bbrc.2004.12.148
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN: 0006-291X Impact factor: 3.575