Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 CUG repeats present in myotonin kinase RNA form metastable "slippery" hairpins.

Literature DB >> 9388259

CUG repeats present in myotonin kinase RNA form metastable "slippery" hairpins.

Abstract

We show that CUG repeats form "slippery" hairpins in their natural sequence context of the myotonin kinase gene transcript. This novel type of RNA structure is characterized by strong S1 and T1 nuclease and lead cleavages in the terminal loop and by mild lead cleavages in the hairpin stem. The latter effect indicates a relaxed metastable structure of the stem. (CUG)5 repeats do not form any detectable secondary structure, whereas hairpins of increasing stability are formed by (CUG)11, (CUG)21, and (CUG)49. The potential role of the RNA hairpin structure in the pathogenesis of myotonic dystrophy is discussed.

Entities: Disease

Mesh：

Substances：

Year: 1997 PMID： 9388259 DOI： 10.1074/jbc.272.49.31079

Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157

Keyword Cloud
Cited

73 in total

1. RNA structure analysis assisted by capillary electrophoresis.

Authors: Krzysztof Sobczak; Wlodzimierz J Krzyzosiak
Journal: Nucleic Acids Res Date: 2002-11-15 Impact factor: 16.971

2. Expansion of the (CTG)(n) repeat in the 5'-UTR of a reporter gene impedes translation.

Authors: G Raca; E Y Siyanova; C T McMurray; S M Mirkin
Journal: Nucleic Acids Res Date: 2000-10-15 Impact factor: 16.971

3. The fragile X syndrome repeats form RNA hairpins that do not activate the interferon-inducible protein kinase, PKR, but are cut by Dicer.

Authors: Vaishali Handa; Tapas Saha; Karen Usdin
Journal: Nucleic Acids Res Date: 2003-11-01 Impact factor: 16.971

CUG repeats present in myotonin kinase RNA form metastable "slippery" hairpins.

1. RNA structure analysis assisted by capillary electrophoresis.

2. Expansion of the (CTG)(n) repeat in the 5'-UTR of a reporter gene impedes translation.

3. The fragile X syndrome repeats form RNA hairpins that do not activate the interferon-inducible protein kinase, PKR, but are cut by Dicer.

Review 4. Structures of trinucleotide repeats in human transcripts and their functional implications.

5. Homonuclear chemical shift correlation in rotating solids via RNnu n symmetry-based adiabatic RF pulse schemes.

6. Characterisation of hydrogen bonding networks in RNAs via magic angle spinning solid state NMR spectroscopy.

7. In vivo co-localisation of MBNL protein with DMPK expanded-repeat transcripts.

8. Dystrophia myotonia: why focus on foci?

Review 9. Pathogenic mechanisms of myotonic dystrophy.

10. Reversal of RNA dominance by displacement of protein sequestered on triplet repeat RNA.