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Literature DB >> 9384277

DNA restriction fragments length polymorphism of Mycobacterium tuberculosis isolates in Pisa, Italy.

C Garzelli¹, N Lari, B Nguon, M Pistello, G Falcone.

Abstract

A total of 60 Mycobacterium tuberculosis strains isolated in the area of Pisa, Italy, over a period from April 1993 to December 1995, were analyzed for the IS6110-based restriction fragments length polymorphism (RFLP). Isolates were found to show a great heterogeneity and only few isolates shared identical DNA banding patterns. In particular, 55 distinct IS6110 patterns were found (average number of isolates per pattern: 1.09) and only 9 strains (15%) occurred in 4 clusters of 2-3 identical clones. Computer analysis of genetic similarities among the strains revealed a family of 17 isolates including the clustered clones implicated in recently acquired infections. No correlation was found between the RFLP DNA patterns of the isolates and drug susceptibility. Of the 5 isolates from immigrants only one showed abnormal DNA fingerprinting. Our data indicate that the patterns of M. tuberculosis isolates in Pisa area are comparable to those of countries with low-prevalence TB and that a low level of TB transmission occurs in this area.

Entities: Chemical Disease Species

Mesh：

Substances：
DNA, Bacterial

Year: 1997 PMID： 9384277 DOI： 10.1023/a:1007337319454

Source DB: PubMed Journal: Eur J Epidemiol ISSN： 0393-2990 Impact factor: 8.082

15 in total

1. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology.

Authors: J D van Embden; M D Cave; J T Crawford; J W Dale; K D Eisenach; B Gicquel; P Hermans; C Martin; R McAdam; T M Shinnick
Journal: J Clin Microbiol Date: 1993-02 Impact factor: 5.948

2. Evidence of nosocomial transmission of tuberculosis among AIDS patients by DNA fingerprinting.

Authors: C Garzelli; N Lari; B Nguon; G Falcone
Journal: New Microbiol Date: 1996-10 Impact factor: 2.479

3. Transmission of tuberculosis in New York City. An analysis by DNA fingerprinting and conventional epidemiologic methods.

Authors: D Alland; G E Kalkut; A R Moss; R A McAdam; J A Hahn; W Bosworth; E Drucker; B R Bloom
Journal: N Engl J Med Date: 1994-06-16 Impact factor: 91.245

4. Analysis of the population structure of Mycobacterium tuberculosis in Ethiopia, Tunisia, and The Netherlands: usefulness of DNA typing for global tuberculosis epidemiology.

Authors: P W Hermans; F Messadi; H Guebrexabher; D van Soolingen; P E de Haas; H Heersma; H de Neeling; A Ayoub; F Portaels; D Frommel
Journal: J Infect Dis Date: 1995-06 Impact factor: 5.226

5. An outbreak of tuberculosis with accelerated progression among persons infected with the human immunodeficiency virus. An analysis using restriction-fragment-length polymorphisms.

Authors: C L Daley; P M Small; G F Schecter; G K Schoolnik; R A McAdam; W R Jacobs; P C Hopewell
Journal: N Engl J Med Date: 1992-01-23 Impact factor: 91.245

6. The epidemiology of tuberculosis in San Francisco. A population-based study using conventional and molecular methods.

Authors: P M Small; P C Hopewell; S P Singh; A Paz; J Parsonnet; D C Ruston; G F Schecter; C L Daley; G K Schoolnik
Journal: N Engl J Med Date: 1994-06-16 Impact factor: 91.245

Review 7. HIV-associated tuberculosis in developing countries: epidemiology and strategies for prevention.

Authors: J P Narain; M C Raviglione; A Kochi
Journal: Tuber Lung Dis Date: 1992-12

8. Insertion element IS986 from Mycobacterium tuberculosis: a useful tool for diagnosis and epidemiology of tuberculosis.

Authors: P W Hermans; D van Soolingen; J W Dale; A R Schuitema; R A McAdam; D Catty; J D van Embden
Journal: J Clin Microbiol Date: 1990-09 Impact factor: 5.948

9. Restriction fragment length polymorphism Mycobacterium tuberculosis strains isolated from Greenland during 1992: evidence of tuberculosis transmission between Greenland and Denmark.

Authors: Z H Yang; P E de Haas; D van Soolingen; J D van Embden; A B Andersen
Journal: J Clin Microbiol Date: 1994-12 Impact factor: 5.948