BACKGROUND: Taxol is a natural product produced by the Pacific yew, Taxus brevifolia, that has emerged as a prominent chemotherapeutic agent for the treatment of solid tumors. It binds to microtubules, stabilizing them and arresting cells in mitosis. Taxol has been produced synthetically and a wealth of structure-activity data has recently emerged. To data, however, no single conformational model exists for the interpretation of these data. Studies of taxol and its analogs in organic solvents showed two distinct conformations, one in which the 3'-benzamido group and the 2-benzoyl group are in close proximity, and another in which the 2-benzoyl group is instead close to the 3'-phenyl group. We decided to use a derivative of taxol that has improved water-solubility to determine the structure of taxol in water. RESULTS: We have synthesized and characterized a stable water-soluble derivative of taxol that binds to microtubules and has a cytotoxicity profile very similar to that of taxol. 1D and 2D 1H NMR experiments with this bioactive compound in D2O indicate the presence of one conformer with a well-defined structure. In this structure, the 2-benzoyl group is clustered with the 3'-phenyl group. CONCLUSION: The determination of the conformation of taxol in water may allow quantitative three-dimensional interpretation of the structure-activity data obtained for taxol, and hence enable the design of novel taxol mimics.
BACKGROUND:Taxol is a natural product produced by the Pacific yew, Taxus brevifolia, that has emerged as a prominent chemotherapeutic agent for the treatment of solid tumors. It binds to microtubules, stabilizing them and arresting cells in mitosis. Taxol has been produced synthetically and a wealth of structure-activity data has recently emerged. To data, however, no single conformational model exists for the interpretation of these data. Studies of taxol and its analogs in organic solvents showed two distinct conformations, one in which the 3'-benzamido group and the 2-benzoyl group are in close proximity, and another in which the 2-benzoyl group is instead close to the 3'-phenyl group. We decided to use a derivative of taxol that has improved water-solubility to determine the structure of taxol in water. RESULTS: We have synthesized and characterized a stable water-soluble derivative of taxol that binds to microtubules and has a cytotoxicity profile very similar to that of taxol. 1D and 2D 1H NMR experiments with this bioactive compound in D2O indicate the presence of one conformer with a well-defined structure. In this structure, the 2-benzoyl group is clustered with the 3'-phenyl group. CONCLUSION: The determination of the conformation of taxol in water may allow quantitative three-dimensional interpretation of the structure-activity data obtained for taxol, and hence enable the design of novel taxol mimics.
Authors: Thota Ganesh; Chao Yang; Andrew Norris; Tom Glass; Susan Bane; Rudravajhala Ravindra; Abhijit Banerjee; Belhu Metaferia; Shala L Thomas; Paraskevi Giannakakou; Ana A Alcaraz; Ami S Lakdawala; James P Snyder; David G I Kingston Journal: J Med Chem Date: 2007-01-31 Impact factor: 7.446
Authors: Younkee Paik; Chao Yang; Belhu Metaferia; Shoubin Tang; Susan Bane; Rudravajhala Ravindra; Natasha Shanker; Ana A Alcaraz; Scott A Johnson; Jacob Schaefer; Robert D O'Connor; Lynette Cegelski; James P Snyder; David G I Kingston Journal: J Am Chem Soc Date: 2007-01-17 Impact factor: 15.419
Authors: Shoubin Tang; Chao Yang; Peggy Brodie; Susan Bane; Rudravajhala Ravindra; Shubhada Sharma; Yi Jiang; James P Snyder; David G I Kingston Journal: Org Lett Date: 2006-08-31 Impact factor: 6.005
Authors: Mathis Hodge; Qiao-Hong Chen; Susan Bane; Shubhada Sharma; Maura Loew; Abhijit Banerjee; Ana A Alcaraz; James P Snyder; David G I Kingston Journal: Bioorg Med Chem Lett Date: 2009-03-21 Impact factor: 2.823