Literature DB >> 9358188

Isolation of nuclei for chromatin analysis in fission yeast.

J A Mason1, J Mellor.   

Abstract

The methods available for analysis of the chromatin of Schizosaccharomyces pombe are time consuming (>8 h) and/or result in some degradation of the chromatin. Here we report an optimised method for the preparation of spheroplasts and the isolation of nuclei which takes <25 min and is suitable for analysis of chromatin structure by micrococcal nuclease, restriction endonuclease or by immunoprecipitation.

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Year:  1997        PMID： 9358188      PMCID： PMC147094          DOI： 10.1093/nar/25.22.4700

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  8 in total

1.  Rapid isolation of yeast plasmids as native chromatin.

Authors:  C Martinez-Campa; N A Kent; J Mellor
Journal:  Nucleic Acids Res       Date:  1997-05-01       Impact factor: 16.971

2.  Chromatin structure snap-shots: rapid nuclease digestion of chromatin in yeast.

Authors:  N A Kent; J Mellor
Journal:  Nucleic Acids Res       Date:  1995-09-25       Impact factor: 16.971

3.  Chromatin analysis in yeast using NP-40 permeabilised sphaeroplasts.

Authors:  N A Kent; L E Bird; J Mellor
Journal:  Nucleic Acids Res       Date:  1993-09-25       Impact factor: 16.971

4.  Molecular genetic analysis of fission yeast Schizosaccharomyces pombe.

Authors:  S Moreno; A Klar; P Nurse
Journal:  Methods Enzymol       Date:  1991       Impact factor: 1.600

5.  Global alterations in chromatin accessibility associated with loss of SIN4 function.

Authors:  T Macatee; Y W Jiang; D J Stillman; S Y Roth
Journal:  Nucleic Acids Res       Date:  1997-03-15       Impact factor: 16.971

6.  A low copy number central sequence with strict symmetry and unusual chromatin structure in fission yeast centromere.

Authors:  K Takahashi; S Murakami; Y Chikashige; H Funabiki; O Niwa; M Yanagida
Journal:  Mol Biol Cell       Date:  1992-07       Impact factor: 4.138

7.  Chromatin structure of Schizosaccharomyces pombe. A nucleosome repeat length that is shorter than the chromatosomal DNA length.

Authors:  J S Godde; J Widom
Journal:  J Mol Biol       Date:  1992-08-20       Impact factor: 5.469

8.  The chromatin structure of centromeres from fission yeast: differentiation of the central core that correlates with function.

Authors:  C Polizzi; L Clarke
Journal:  J Cell Biol       Date:  1991-01       Impact factor: 10.539
  8 in total
  3 in total

1.  Schizosaccharomyces pombe minichromosome maintenance-binding protein (MCM-BP) antagonizes MCM helicase.

Authors:  Lin Ding; Susan L Forsburg
Journal:  J Biol Chem       Date:  2011-08-03       Impact factor: 5.157

2.  CHD1 remodelers regulate nucleosome spacing in vitro and align nucleosomal arrays over gene coding regions in S. pombe.

Authors:  Julia Pointner; Jenna Persson; Punit Prasad; Ulrika Norman-Axelsson; Annelie Strålfors; Olga Khorosjutina; Nils Krietenstein; J Peter Svensson; Karl Ekwall; Philipp Korber
Journal:  EMBO J       Date:  2012-10-26       Impact factor: 11.598

3.  Integrity of chromatin and replicating DNA in nuclei released from fission yeast by semi-automated grinding in liquid nitrogen.

Authors:  Robert M Givens; Larry D Mesner; Joyce L Hamlin; Michael J Buck; Joel A Huberman
Journal:  BMC Res Notes       Date:  2011-11-16
  3 in total

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