Literature DB >> 9358176

Increased DNA binding and sequence discrimination of PNA oligomers containing 2,6-diaminopurine.

G Haaima1, H F Hansen, L Christensen, O Dahl, P E Nielsen.   

Abstract

The synthesis of a diaminopurine PNA monomer, N-[N6-(benzyloxycarbonyl)-2,6-diaminopurine-9-yl] acetyl-N-(2-t-butyloxycarbonylaminoethyl)glycine, and the incorporation of this monomer into PNA oligomers are described. Substitution of adenine by diaminopurine in PNA oligomers increased the T m of duplexes formed with complementary DNA, RNA or PNA by 2.5-6.5 degrees C per diaminopurine. Furthermore, discrimination against mismatches facing the diaminopurine in the hybridizing oligomer is improved. Finally, a homopurine decamer PNA containing six diaminopurines is shown to form a (gel shift) stable strand displacement complex with a target in a 246 bp double-stranded DNA fragment.

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Year:  1997        PMID： 9358176      PMCID： PMC147079          DOI： 10.1093/nar/25.22.4639

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  15 in total

1.  Solid-phase synthesis of peptide nucleic acids.

Authors:  L Christensen; R Fitzpatrick; B Gildea; K H Petersen; H F Hansen; T Koch; M Egholm; O Buchardt; P E Nielsen; J Coull; R H Berg
Journal:  J Pept Sci       Date:  1995 May-Jun       Impact factor: 1.905

2.  Antisense and antigene properties of peptide nucleic acids.

Authors:  J C Hanvey; N J Peffer; J E Bisi; S A Thomson; R Cadilla; J A Josey; D J Ricca; C F Hassman; M A Bonham; K G Au
Journal:  Science       Date:  1992-11-27       Impact factor: 47.728

3.  PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules.

Authors:  M Egholm; O Buchardt; L Christensen; C Behrens; S M Freier; D A Driver; R H Berg; S K Kim; B Norden; P E Nielsen
Journal:  Nature       Date:  1993-10-07       Impact factor: 49.962

4.  Screening for genetic mutations.

Authors:  C Carlsson; M Jonsson; B Nordén; M T Dulay; R N Zare; J Noolandi; P E Nielsen; L C Tsui; J Zielenski
Journal:  Nature       Date:  1996-03-21       Impact factor: 49.962

5.  2-aminoadenine is an adenine substituting for a base in S-2L cyanophage DNA.

Authors:  M D Kirnos; I Y Khudyakov; N I Alexandrushkina; B F Vanyushin
Journal:  Nature       Date:  1977-11-24       Impact factor: 49.962

6.  Antisense properties of duplex- and triplex-forming PNAs.

Authors:  H Knudsen; P E Nielsen
Journal:  Nucleic Acids Res       Date:  1996-02-01       Impact factor: 16.971

7.  An assessment of the antisense properties of RNase H-competent and steric-blocking oligomers.

Authors:  M A Bonham; S Brown; A L Boyd; P H Brown; D A Bruckenstein; J C Hanvey; S A Thomson; A Pipe; F Hassman; J E Bisi
Journal:  Nucleic Acids Res       Date:  1995-04-11       Impact factor: 16.971

8.  Sequence-specific purification of nucleic acids by PNA-controlled hybrid selection.

Authors:  H Orum; P E Nielsen; M Jørgensen; C Larsson; C Stanley; T Koch
Journal:  Biotechniques       Date:  1995-09       Impact factor: 1.993

9.  Efficient pH-independent sequence-specific DNA binding by pseudoisocytosine-containing bis-PNA.

Authors:  M Egholm; L Christensen; K L Dueholm; O Buchardt; J Coull; P E Nielsen
Journal:  Nucleic Acids Res       Date:  1995-01-25       Impact factor: 16.971

10.  Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide.

Authors:  P E Nielsen; M Egholm; R H Berg; O Buchardt
Journal:  Science       Date:  1991-12-06       Impact factor: 47.728
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  21 in total

1.  Double duplex invasion by peptide nucleic acid: a general principle for sequence-specific targeting of double-stranded DNA.

Authors:  J Lohse; O Dahl; P E Nielsen
Journal:  Proc Natl Acad Sci U S A       Date:  1999-10-12       Impact factor: 11.205

2.  Kinetics and mechanism of the DNA double helix invasion by pseudocomplementary peptide nucleic acids.

Authors:  Vadim V Demidov; Ekaterina Protozanova; Konstantin I Izvolsky; Christopher Price; Peter E Nielsen; Maxim D Frank-Kamenetskii
Journal:  Proc Natl Acad Sci U S A       Date:  2002-04-23       Impact factor: 11.205

3.  Pyrazolo[3,4-d]pyrimidine nucleic acids: adjustment of dA-dT to dG-dC base pair stability.

Authors:  F Seela; G Becher
Journal:  Nucleic Acids Res       Date:  2001-05-15       Impact factor: 16.971

4.  siRNA function in RNAi: a chemical modification analysis.

Authors:  Ya-Lin Chiu; Tariq M Rana
Journal:  RNA       Date:  2003-09       Impact factor: 4.942

5.  Propynyl groups in duplex DNA: stability of base pairs incorporating 7-substituted 8-aza-7-deazapurines or 5-substituted pyrimidines.

Authors:  Junlin He; Frank Seela
Journal:  Nucleic Acids Res       Date:  2002-12-15       Impact factor: 16.971

Review 6.  PNA Technology.

Authors:  Peter E Nielsen
Journal:  Mol Biotechnol       Date:  2004-03       Impact factor: 2.695

7.  Site-selective and hydrolytic two-strand scission of double-stranded DNA using Ce(IV)/EDTA and pseudo-complementary PNA.

Authors:  Yoji Yamamoto; Akihiko Uehara; Takafumi Tomita; Makoto Komiyama
Journal:  Nucleic Acids Res       Date:  2004-11-01       Impact factor: 16.971

8.  A phenomenological model for predicting melting temperatures of DNA sequences.

Authors:  Garima Khandelwal; Jayaram Bhyravabhotla
Journal:  PLoS One       Date:  2010-08-26       Impact factor: 3.240

Review 9.  Chemical modifications of artificial restriction DNA cutter (ARCUT) to promote its in vivo and in vitro applications.

Authors:  Makoto Komiyama
Journal:  Artif DNA PNA XNA       Date:  2014-12-15

10.  Introduction of multiphosphonate ligand to peptide nucleic acid for metal ion conjugation.

Authors:  Yuichiro Aiba; Yuta Honda; Yue Han; Makoto Komiyama
Journal:  Artif DNA PNA XNA       Date:  2012-04-01
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