Literature DB >> 9336478

A rapid RT-PCR based method to isolate complementary DNA fragments flanking retrovirus integration sites.

P J Valk1, M Joosten, Y Vankan, B Löwenberg, R Delwel.   

Abstract

Proto-oncogenes in retrovirally induced myeloid mouse leukemias are frequently activated following retroviral insertion. The identification of common virus integration sites (VISs) and isolation of the transforming oncogene is laborious and time consuming. We established a rapid and simple PCR based procedure which facilitates the identification of VISs and novel proto-oncogenes. Complementary DNA fragments adjacent to retrovirus integration sites were selectively isolated by applying a reverse transcriptase (RT) reaction using an oligo(dT)-adaptor primer, followed by PCR using the adaptor sequence and a retrovirus long terminal repeat (LTR) specific primer. Multiple chimeric cDNA fragments suitable for Southern and northern blot analysis were isolated.

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Year:  1997        PMID： 9336478      PMCID： PMC147027          DOI： 10.1093/nar/25.21.4419

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  18 in total

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Authors:  J Jonkers; A Berns
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Authors:  M J O'Neill; A H Sinclair
Journal:  Nucleic Acids Res       Date:  1997-07-01       Impact factor: 16.971

3.  Polymerase chain reaction with single-sided specificity: analysis of T cell receptor delta chain.

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4.  Novel use of polymerase chain reaction to amplify cellular DNA adjacent to an integrated provirus.

Authors:  J Silver; V Keerikatte
Journal:  J Virol       Date:  1989-05       Impact factor: 5.103

5.  His-1 and His-2: identification and chromosomal mapping of two commonly rearranged sites of viral integration in a myeloid leukemia.

Authors:  D S Askew; C Bartholomew; A M Buchberg; M B Valentine; N A Jenkins; N G Copeland; J N Ihle
Journal:  Oncogene       Date:  1991-11       Impact factor: 9.867

6.  Transient transfection of ecotropic retrovirus receptor permits stable gene transfer into non-rodent cells with murine retroviral vectors.

Authors:  A Scholz; M Beato
Journal:  Nucleic Acids Res       Date:  1996-03-01       Impact factor: 16.971

7.  Promoter region of mouse Tcrg genes.

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8.  Retroviral activation of a novel gene encoding a zinc finger protein in IL-3-dependent myeloid leukemia cell lines.

Authors:  K Morishita; D S Parker; M L Mucenski; N A Jenkins; N G Copeland; J N Ihle
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9.  Retroviral insertions in the CB-1/Fim-3 common site of integration activate expression of the Evi-1 gene.

Authors:  C Bartholomew; K Morishita; D Askew; A Buchberg; N A Jenkins; N G Copeland; J N Ihle
Journal:  Oncogene       Date:  1989-05       Impact factor: 9.867

10.  Correlation of cell-surface phenotype with the establishment of interleukin 3-dependent cell lines from wild-mouse murine leukemia virus-induced neoplasms.

Authors:  K L Holmes; E Palaszynski; T N Fredrickson; H C Morse; J N Ihle
Journal:  Proc Natl Acad Sci U S A       Date:  1985-10       Impact factor: 11.205
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Authors:  P J Valk; Y Vankan; M Joosten; N A Jenkins; N G Copeland; B Löwenberg; R Delwel
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4.  K1 protein of human herpesvirus 8 suppresses lymphoma cell Fas-mediated apoptosis.

Authors:  Suizhao Wang; Shu Wang; Hoyoung Maeng; Daniel P Young; Om Prakash; Luis E Fayad; Anas Younes; Felipe Samaniego
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5.  RTCGD: retroviral tagged cancer gene database.

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  5 in total

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