| Literature DB >> 9327399 |
E Mine1, H Sakurai, S Izumi, S Tomino.
Abstract
This article describes the development of cell-free transcription systems from the cultured cells and fat body tissues of a Lepidpteran insect, the silkworm, Bombyx mori. Detailed protocols are provided for the culture of a B. mori cell line, rearing larvae, preparation of whole cell as well as nuclear extract and conditions for in vitro transcription of cloned plasma protein gene templates.Entities:
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Year: 1997 PMID: 9327399 DOI: 10.1007/BF02762341
Source DB: PubMed Journal: Mol Biotechnol ISSN: 1073-6085 Impact factor: 2.695