Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Development of a method for direct quantification of cytomegalovirus antigenemia by flow cytometry.

Literature DB >> 9316930

Development of a method for direct quantification of cytomegalovirus antigenemia by flow cytometry.

B M Imbert-Marcille¹, N Robillard, A S Poirier, M Coste-Burel, D Cantarovich, N Milpied, S Billaudel.

Abstract

Cytomegalovirus (CMV) antigenemia was directly detected in polymorphonuclear leukocytes (PMNLs) from transplant recipients by using flow cytometry (FC). Two fixation and permeabilization methods and seven anti-CMV monoclonal antibodies (MAbs) were evaluated. 1C3, SL20, and NEA-9221 MAbs were more efficacious. The antigenemia detection threshold of FC was 0.05% positive PMNLs, and percentages correlated well with DNA viral load and the appearance of clinical symptoms.

Entities: Disease

Mesh：

Substances：

Year: 1997 PMID： 9316930 PMCID： PMC230033 DOI： 10.1128/jcm.35.10.2665-2669.1997

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

20 in total

Review 1. Direct detection of cytomegalovirus in peripheral blood leukocytes--a review of the antigenemia assay and polymerase chain reaction.

Authors: T H The; M van der Ploeg; A P van den Berg; A M Vlieger; M van der Giessen; W J van Son
Journal: Transplantation Date: 1992-08 Impact factor: 4.939

Review 2. Uses of flow cytometry in virology.

Authors: J J McSharry
Journal: Clin Microbiol Rev Date: 1994-10 Impact factor: 26.132

3. Automated image cytometry for detection of rare, viral antigen-positive cells in peripheral blood.

Authors: J J Ploem-Zaaijer; W E Mesker; G J Boland; W C Sloos; F M van de Rijke; M Jiwa; A K Raap
Journal: Cytometry Date: 1994-03-01

4. A gentle fixation and permeabilization method for combined cell surface and intracellular staining with improved precision in DNA quantification.

Authors: I Schmid; C H Uittenbogaart; J V Giorgi
Journal: Cytometry Date: 1991

5. Quantification of low frequency white blood cells expressing human cytomegalovirus antigen by image cytometry.

Authors: N M Jiwa; W E Mesker; J J Ploem-Zaaijer; W van Dorp; T H The; A K Raap
Journal: Cytometry Date: 1994-05-01

6. Sequential paraformaldehyde and methanol fixation for simultaneous flow cytometric analysis of DNA, cell surface proteins, and intracellular proteins.

Authors: A A Pollice; J P McCoy; S E Shackney; C A Smith; J Agarwal; D R Burholt; L E Janocko; F J Hornicek; S G Singh; R J Hartsock
Journal: Cytometry Date: 1992

7. Monitoring of human cytomegalovirus infections and ganciclovir treatment in heart transplant recipients by determination of viremia, antigenemia, and DNAemia.

Authors: G Gerna; D Zipeto; M Parea; M G Revello; E Silini; E Percivalle; M Zavattoni; P Grossi; G Milanesi
Journal: J Infect Dis Date: 1991-09 Impact factor: 5.226

8. Comparison of quantitative cytomegalovirus antigenemia assay with culture methods and correlation with clinical disease.

Authors: M L Landry; D Ferguson
Journal: J Clin Microbiol Date: 1993-11 Impact factor: 5.948

9. Clinical and practical value of human cytomegalovirus DNAemia detection by semi-nested PCR for follow-up of BMT recipients.

Authors: B M Imbert-Marcille; N Milpied; M Coste-Burel; B Richet; P Moreau; J L Harousseau; S Billaudel
Journal: Bone Marrow Transplant Date: 1995-04 Impact factor: 5.483

10. Comparison of different immunostaining techniques and monoclonal antibodies to the lower matrix phosphoprotein (pp65) for optimal quantitation of human cytomegalovirus antigenemia.

Authors: G Gerna; M G Revello; E Percivalle; F Morini
Journal: J Clin Microbiol Date: 1992-05 Impact factor: 5.948

8 in total

1. Clinical relevance of direct quantification of pp65 antigenemia using flow cytometry in solid organ and stem cell transplant recipients.

Authors: A S Poirier-Toulemonde; N Milpied; D Cantarovich; J F Morcet; S Billaudel; B M Imbert-Marcille
Journal: J Clin Microbiol Date: 2000-09 Impact factor: 5.948

2. Sequential use of paraformaldehyde and methanol as optimal conditions for the direct quantification of ZEBRA and rta antigens by flow cytometry.

Authors: B M Imbert-Marcille; M Coste-Burel; N Robillard; J Foucaud-Gamen; S Billaudel; E Drouet
Journal: Clin Diagn Lab Immunol Date: 2000-03

3. Flow cytometry compared with indirect immunofluorescence for rapid detection of dengue virus type 1 after amplification in tissue culture.

Authors: C L Kao; M C Wu; Y H Chiu; J L Lin; Y C Wu; Y Y Yueh; L K Chen; M F Shaio; C C King
Journal: J Clin Microbiol Date: 2001-10 Impact factor: 5.948

4. Generation of cytomegalovirus-specific human T-lymphocyte clones by using autologous B-lymphoblastoid cells with stable expression of pp65 or IE1 proteins: a tool to study the fine specificity of the antiviral response.

Authors: C Retière; V Prod'homme; B M Imbert-Marcille; M Bonneville; H Vié; M M Hallet
Journal: J Virol Date: 2000-05 Impact factor: 5.103

8. Diagnostic value of HCMV pp65 antigen detection by FCA for symptomatic and asymptomatic infection: compared to quantification of HCMV DNA and detection of IgM antibody in infants.

Authors: Zhengrong Sun; Xiangshi Ceng; Zhiqin Mao; Jidong Wang; Qing Liu; Yaohua Ji; Yanping Ma; Ying Qi; Rong He; Qiang Ruan
Journal: Med Microbiol Immunol Date: 2009-03-24 Impact factor: 3.402