J Sugawara1, T Fukaya, T Murakami, H Yoshida, A Yajima. 1. Department of Obstetrics and Gynecology, School of Medicine, Tohoku University, Sendai, Japan. SUGAWARA@OB-GY.MED.TOHOKU.AC.JP
Abstract
OBJECTIVE: We evaluated the secretion of hepatocyte growth factor by eutopic endometrial stromal cells in vitro. DESIGN: Eutopic endometrial stromal cells were isolated and the culture supernatants were collected after 48 hours of incubation. The secretion of hepatocyte growth factor was analyzed by ELISA. SETTING: Department of Obstetrics and Gynecology of Tohoku University Hospital. PATIENT(S): Specimens of endometrium in the proliferative phase were excised from 11 patients who were undergoing laparoscopy for infertility. Six of the patients had endometriosis and five did not. None of the patients had received hormonal treatment before surgery. RESULT(S): Hepatocyte growth factor secretion was found to be increased significantly in cultured endometrial stromal cells from the infertile patients with endometriosis compared with those without endometriosis. CONCLUSION(S): The secretion of hepatocyte growth factor was up-regulated in eutopic endometrial stromal cells from patients with endometriosis. An increase in hepatocyte growth factor production may be characteristic of endometriosis and may be involved in the pathophysiology of this disease.
OBJECTIVE: We evaluated the secretion of hepatocyte growth factor by eutopic endometrial stromal cells in vitro. DESIGN: Eutopic endometrial stromal cells were isolated and the culture supernatants were collected after 48 hours of incubation. The secretion of hepatocyte growth factor was analyzed by ELISA. SETTING: Department of Obstetrics and Gynecology of Tohoku University Hospital. PATIENT(S): Specimens of endometrium in the proliferative phase were excised from 11 patients who were undergoing laparoscopy for infertility. Six of the patients had endometriosis and five did not. None of the patients had received hormonal treatment before surgery. RESULT(S): Hepatocyte growth factor secretion was found to be increased significantly in cultured endometrial stromal cells from the infertilepatients with endometriosis compared with those without endometriosis. CONCLUSION(S): The secretion of hepatocyte growth factor was up-regulated in eutopic endometrial stromal cells from patients with endometriosis. An increase in hepatocyte growth factor production may be characteristic of endometriosis and may be involved in the pathophysiology of this disease.
Authors: Jean Bouquet De Jolinière; Jean Marc Bernard Ayoubi; Luca Gianaroli; Jean Bernard Dubuisson; Jean Gogusev; Anis Feki Journal: Front Surg Date: 2014-05-27
Authors: Margherita Y Turco; Lucy Gardner; Jasmine Hughes; Tereza Cindrova-Davies; Maria J Gomez; Lydia Farrell; Michael Hollinshead; Steven G E Marsh; Jan J Brosens; Hilary O Critchley; Benjamin D Simons; Myriam Hemberger; Bon-Kyoung Koo; Ashley Moffett; Graham J Burton Journal: Nat Cell Biol Date: 2017-04-10 Impact factor: 28.824