Literature DB >> 9302215

Rapid identification of rough Brucella isolates by a latex coagglutination assay with the 25-kilodalton outer membrane protein and rough-lipopolysaccharide-specific monoclonal antibodies.

R A Bowden1, J M Verger, M Grayon, A Cloeckaert.   

Abstract

A latex coagglutination assay was developed to identify rough (R) isolates of Brucella. Latex beads were coated, via protein A, with either an anti-Brucella rough-lipopolysaccharide (R-LPS) monoclonal antibody (MAb) or an anti-Brucella 25-kDa outer membrane protein (Omp25) MAb. Slide agglutination tests were done for 68 strains of Brucella spp., including type strains of all biovars as well as field isolates. Latex beads coated with MAb to R-LPS coagglutinated only R strains, whereas latex beads coated with MAb to Omp25 coagglutinated all the R Brucella isolates except Brucella ovis. Coagglutination was easier to read than agglutination with rabbit R-Brucella-specific antiserum. Thus, this assay accurately differentiates B. ovis from other R Brucella isolates. The latex coagglutination assay can substitute, to advantage, for the current anti-Brucella (R) rabbit monospecific serum.

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Year:  1997        PMID： 9302215      PMCID： PMC170608          DOI： 10.1128/cdli.4.5.611-614.1997

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  9 in total

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Authors:  G G ALTON
Journal:  J Comp Pathol       Date:  1960-01       Impact factor: 1.311

2.  Purification and antigenic analysis of the major 25-kilodalton outer membrane protein of Brucella abortus.

Authors:  A Cloeckaert; M S Zygmunt; G Bézard; G Dubray
Journal:  Res Microbiol       Date:  1996-05       Impact factor: 3.992

3.  Nucleotide sequence and expression of the gene encoding the major 25-kilodalton outer membrane protein of Brucella ovis: Evidence for antigenic shift, compared with other Brucella species, due to a deletion in the gene.

Authors:  A Cloeckaert; J M Verger; M Grayon; M S Zygmunt; O Grépinet
Journal:  Infect Immun       Date:  1996-06       Impact factor: 3.441

4.  Identification of seven surface-exposed Brucella outer membrane proteins by use of monoclonal antibodies: immunogold labeling for electron microscopy and enzyme-linked immunosorbent assay.

Authors:  A Cloeckaert; P de Wergifosse; G Dubray; J N Limet
Journal:  Infect Immun       Date:  1990-12       Impact factor: 3.441

5.  Specificity of six gene sequences for the detection of the genus Brucella by DNA amplification.

Authors:  M Da Costa; J P Guillou; B Garin-Bastuji; M Thiébaud; G Dubray
Journal:  J Appl Bacteriol       Date:  1996-09

6.  Surface exposure of outer membrane protein and lipopolysaccharide epitopes in Brucella species studied by enzyme-linked immunosorbent assay and flow cytometry.

Authors:  R A Bowden; A Cloeckaert; M S Zygmunt; S Bernard; G Dubray
Journal:  Infect Immun       Date:  1995-10       Impact factor: 3.441

7.  Monoclonal antibodies to Brucella rough lipopolysaccharide: characterization and evaluation of their protective effect against B. abortus.

Authors:  A Cloeckaert; I Jacques; R A Bowden; G Dubray; J N Limet
Journal:  Res Microbiol       Date:  1993 Jul-Aug       Impact factor: 3.992

8.  A rapid and sensitive method for the identification of Brucella species with a monoclonal antibody.

Authors:  N Vizcaíno; L Fernández-Lago
Journal:  Res Microbiol       Date:  1992-06       Impact factor: 3.992

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Journal:  J Clin Microbiol       Date:  1987-11       Impact factor: 5.948
  9 in total
  3 in total

1.  Latex agglutination using the periplasmic proteins antigen of Brucella melitensis is a successful, rapid, and specific serodiagnostic test for ovine brucellosis.

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3.  Evaluation of Reactivity of Monoclonal Antibodies Against Omp25 of Brucella spp.

Authors:  Xin Yang; Zuoping He; Guoxia Zhang; Jinhui Lu; Hui Zhang; Hui Ren; Yanjun Tian; Heng Yang; Chuangfu Chen; Linhai Li; Yongshui Fu; Jean-Pierre Allain; Chengyao Li; Wenjing Wang
Journal:  Front Cell Infect Microbiol       Date:  2020-04-21       Impact factor: 5.293
  3 in total

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