Literature DB >> 9300381

Detection of BK virus in urine by polymerase chain reaction: a comparison of DNA extraction methods.

A Behzadbehbahani1, P E Klapper, P J Vallely, G M Cleator.   

Abstract

Using specimens spiked with BK virus, several DNA extraction methods were evaluated for their ability to remove polymerase chain reaction (PCR) inhibitors from urine samples. It was found that PCR inhibition could be completely overcome by extracting samples with 30% polyethylene glycol (PEG) in 3 M sodium chloride, and partially overcome by extracting samples with guanidine thiocyanate in the presence of high salt concentrations. The nature of the sample inhibition was investigated, leading to the conclusion that both urea and unidentified non-proteinaceous DNA associated substances inhibit BKV DNA amplification from urine.

Entities:  

Mesh：

Substances：

Year:  1997        PMID： 9300381     DOI： 10.1016/s0166-0934(97)00101-8

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  12 in total

1.  Simple method for DNA extraction from pancreatic juice for PCR amplification assays.

Authors:  P Müller; R Jesnowski; S Liebe; A Rolfs; M Löhr
Journal:  Int J Pancreatol       Date:  1999-02

2.  PCR and restriction endonuclease analysis for rapid identification of human adenovirus subgenera.

Authors:  E M Elnifro; R J Cooper; P E Klapper; A S Bailey
Journal:  J Clin Microbiol       Date:  2000-06       Impact factor: 5.948

3.  Comparative evaluation of three commercial systems for nucleic acid extraction from urine specimens.

Authors:  Yi-Wei Tang; Susan E Sefers; Haijing Li; Debra J Kohn; Gary W Procop
Journal:  J Clin Microbiol       Date:  2005-09       Impact factor: 5.948

4.  Critical evaluation of urine-based PCR assay for diagnosis of Lyme borreliosis.

Authors:  Carolin Rauter; Markus Mueller; Isabel Diterich; Sabine Zeller; Dieter Hassler; Thomas Meergans; Thomas Hartung
Journal:  Clin Diagn Lab Immunol       Date:  2005-08

5.  Comparison of various sample preparation methods for PCR diagnosis of visceral leishmaniasis using peripheral blood.

Authors:  L Lachaud; E Chabbert; P Dubessay; J Reynes; J Lamothe; P Bastien
Journal:  J Clin Microbiol       Date:  2001-02       Impact factor: 5.948

6.  Loop-mediated isothermal amplification test for detection of Neisseria gonorrhoeae in urine samples and tolerance of the assay to the presence of urea.

Authors:  Thomas Edwards; Patricia A Burke; Helen B Smalley; Liz Gillies; Glyn Hobbs
Journal:  J Clin Microbiol       Date:  2014-03-12       Impact factor: 5.948

7.  Use of 18S rRNA gene-based PCR assay for diagnosis of acanthamoeba keratitis in non-contact lens wearers in India.

Authors:  Gunisha Pasricha; Savitri Sharma; Prashant Garg; Ramesh K Aggarwal
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948

8.  JC polyomavirus infection in candidates for kidney transplantation living in the Brazilian Amazon region.

Authors:  Fernando Assis Ferreira Melo; Ana Caroline Fonseca Bezerra; Bárbara Brasil Santana; Marluísa Oliveira Guimarães Ishak; Ricardo Ishak; Izaura Maria Vieira Cayres-Vallinoto; Antonio Carlos Rosário Vallinoto
Journal:  Mem Inst Oswaldo Cruz       Date:  2013-04       Impact factor: 2.743

9.  Loop-mediated isothermal amplification assay for rapid detection of common strains of Escherichia coli.

Authors:  Joshua Hill; Shilpa Beriwal; Ishwad Chandra; Vinod K Paul; Aarti Kapil; Tripti Singh; Robert M Wadowsky; Vinita Singh; Ankur Goyal; Timo Jahnukainen; James R Johnson; Phillip I Tarr; Abhay Vats
Journal:  J Clin Microbiol       Date:  2008-06-11       Impact factor: 5.948

10.  JC Virus and APOL1 Risk Alleles in Black South Africans With Hypertension-Attributed CKD.

Authors:  Nolubabalo U Nqebelele; Caroline Dickens; Therese Dix-Peek; Raquel Duarte; Saraladevi Naicker
Journal:  Kidney Int Rep       Date:  2019-05-17
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.