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Literature DB >> 9299782

One-step transformation of the dimorphic yeast Yarrowia lipolytica.

Abstract

An efficient one-step transformation method for the dimorphic yeast Yarrowia lipolytica is described. Using cells grown overnight on agar plates, the whole process is carried out within 1 h. The transformant clones could be recovered on selective plates as early as 36-48 h after plating. The efficiency was better than 10(5) transformants/micrograms replicative plasmid DNA. Effects of cell density, dithiothreitol, heat shock, poly(ethylene glycol) 4000 concentration and the wetness of selective plates were investigated.

Entities: Chemical Species

Mesh：

Year: 1997 PMID： 9299782 DOI： 10.1007/s002530051043

Source DB: PubMed Journal: Appl Microbiol Biotechnol ISSN： 0175-7598 Impact factor: 4.813

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Cited

49 in total

1. Tay1 protein, a novel telomere binding factor from Yarrowia lipolytica.

Authors: Juraj Kramara; Smaranda Willcox; Stanislava Gunisova; Slavomir Kinsky; Jozef Nosek; Jack D Griffith; Lubomir Tomaska
Journal: J Biol Chem Date: 2010-10-05 Impact factor: 5.157

2. Functional characterization of extracellular chitinase encoded by the YlCTS1 gene in a dimorphic yeast Yarrowia lipolytica.

Authors: Jeong-Nam Park; Chang Pyo Han; Dong-Jik Lee; Seon Ah Cheon; Hyun Ah Kang
Journal: J Microbiol Date: 2014-03-29 Impact factor: 3.422

3. Cell-surface expression of Aspergillus saitoi-derived functional α-1,2-mannosidase on Yarrowia lipolytica for glycan remodeling.

Authors: Hye Yun Moon; Trinh Luu Van; Seon Ah Cheon; Jinho Choo; Jeong-Yoon Kim; Hyun Ah Kang
Journal: J Microbiol Date: 2013-08-30 Impact factor: 3.422

4. Essential role of YlMPO1, a novel Yarrowia lipolytica homologue of Saccharomyces cerevisiae MNN4, in mannosylphosphorylation of N- and O-linked glycans.

Authors: Jeong-Nam Park; Yunkyoung Song; Seon Ah Cheon; Ohsuk Kwon; Doo-Byoung Oh; Yoshifumi Jigami; Jeong-Yoon Kim; Hyun Ah Kang
Journal: Appl Environ Microbiol Date: 2010-12-23 Impact factor: 4.792

5. Protein production in Yarrowia lipolytica via fusion to the secreted lipase Lip2p.

Authors: Thomas Hofmeyer; Siyavuya Ishmael Bulani; Julius Grzeschik; Simon Krah; Bernhard Glotzbach; Christina Uth; Olga Avrutina; Michael Brecht; Hans Ulrich Göringer; Petrus van Zyl; Harald Kolmar
Journal: Mol Biotechnol Date: 2014-01 Impact factor: 2.695

One-step transformation of the dimorphic yeast Yarrowia lipolytica.

1. Tay1 protein, a novel telomere binding factor from Yarrowia lipolytica.

2. Functional characterization of extracellular chitinase encoded by the YlCTS1 gene in a dimorphic yeast Yarrowia lipolytica.

3. Cell-surface expression of Aspergillus saitoi-derived functional α-1,2-mannosidase on Yarrowia lipolytica for glycan remodeling.

4. Essential role of YlMPO1, a novel Yarrowia lipolytica homologue of Saccharomyces cerevisiae MNN4, in mannosylphosphorylation of N- and O-linked glycans.

5. Protein production in Yarrowia lipolytica via fusion to the secreted lipase Lip2p.

6. Exploring medium-chain-length polyhydroxyalkanoates production in the engineered yeast Yarrowia lipolytica.

7. High efficiency transformation by electroporation of Yarrowia lipolytica.

8. EasyCloneYALI: Toolbox for CRISPR-Mediated Integrations and Deletions in Yarrowia lipolytica.

9. Robust succinic acid production from crude glycerol using engineered Yarrowia lipolytica.

10. Engineering Yarrowia lipolytica as a platform for synthesis of drop-in transportation fuels and oleochemicals.