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Literature DB >> 33847981

EasyCloneYALI: Toolbox for CRISPR-Mediated Integrations and Deletions in Yarrowia lipolytica.

Jonathan Dahlin¹, Carina Holkenbrink¹, Irina Borodina².

Abstract

In order to unlock the full potential of Yarrowia lipolytica, as model organism and production host, simple and reliable tools for genome engineering are essential. In this chapter, the practical details of working with the EasyCloneYALI Toolbox are described.Highlights of the EasyCloneYALI Toolbox are high genome editing efficiencies, multiplexed Cas9-mediated knockouts, targeted genomic integrations into characterized intergenic loci, as well as streamlined and convenient cloning for both marker-based and marker-free integrative expression vectors.

Entities: Species

Keywords: CRISPR; Cas9; Chromosomal integration; EasyClone; EasyCloneYALI; Genome editing; Metabolic engineering

Year: 2021 PMID： 33847981 DOI： 10.1007/978-1-0716-1414-3_3

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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References

10 in total

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EasyCloneYALI: Toolbox for CRISPR-Mediated Integrations and Deletions in Yarrowia lipolytica.

1. One-step transformation of the dimorphic yeast Yarrowia lipolytica.

2. Validating genome-wide CRISPR-Cas9 function improves screening in the oleaginous yeast Yarrowia lipolytica.

3. Increased homologous integration frequency in Yarrowia lipolytica strains defective in non-homologous end-joining.

4. The LYR protein subunit NB4M/NDUFA6 of mitochondrial complex I anchors an acyl carrier protein and is essential for catalytic activity.

5. Efficient homologous recombination with short length flanking fragments in Ku70 deficient Yarrowia lipolytica strains.

6. EasyCloneYALI: CRISPR/Cas9-Based Synthetic Toolbox for Engineering of the Yeast Yarrowia lipolytica.

7. Multiple Parameters Drive the Efficiency of CRISPR/Cas9-Induced Gene Modifications in Yarrowia lipolytica.

8. EasyClone: method for iterative chromosomal integration of multiple genes in Saccharomyces cerevisiae.

9. USER friendly DNA engineering and cloning method by uracil excision.

10. CHOPCHOP v2: a web tool for the next generation of CRISPR genome engineering.