Literature DB >> 9294159

Bacterial resistance to vancomycin: overproduction, purification, and characterization of VanC2 from Enterococcus casseliflavus as a D-Ala-D-Ser ligase.

I S Park1, C H Lin, C T Walsh.   

Abstract

The VanC phenotype for clinical resistance of enterococci to vancomycin is exhibited by Enterococcus gallinarum and Enterococcus casseliflavus. Based on the detection of the cell precursor UDP-N-acetylmuramic acid pentapeptide intermediate terminating in D-Ala-D-Ser instead of D-Ala-D-Ala, it has been predicted that the VanC ligase would be a D-Ala-D-Ser rather than a D-Ala-D-Ala ligase. Overproduction of the E. casseliflavus ATCC 25788 vanC2 gene in Escherichia coli and its purification to homogeneity allowed demonstration of ATP-dependent D-Ala-D-Ser ligase activity. The kcat/Km2 (Km2 = Km for D-Ser or C-terminal D-Ala) ratio for D-Ala-D-Ser/D-Ala-D-Ala dipeptide formation is 270/0.69 for a 400-fold selection against D-Ala in the C-terminal position. VanC2 also has substantial D-Ala-D-Asn ligase activity (kcat/Km2 = 74 mM-1min-1).

Entities:  

Mesh：

Substances：

Year:  1997        PMID： 9294159      PMCID： PMC23297          DOI： 10.1073/pnas.94.19.10040

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  27 in total

1.  Cooperativity and anti-cooperativity between ligand binding and the dimerization of ristocetin A: asymmetry of a homodimer complex and implications for signal transduction.

Authors:  Y R Cho; A J Maguire; A C Try; M S Westwell; P Groves; D H Williams
Journal:  Chem Biol       Date:  1996-03

Review 2.  Bacterial resistance to vancomycin: five genes and one missing hydrogen bond tell the story.

Authors:  C T Walsh; S L Fisher; I S Park; M Prahalad; Z Wu
Journal:  Chem Biol       Date:  1996-01

3.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

4.  Biosynthetic alanine racemase of Salmonella typhimurium: purification and characterization of the enzyme encoded by the alr gene.

Authors:  N Esaki; C T Walsh
Journal:  Biochemistry       Date:  1986-06-03       Impact factor: 3.162

5.  D-alanine:D-alanine ligase: phosphonate and phosphinate intermediates with wild type and the Y216F mutant.

Authors:  C Fan; I S Park; C T Walsh; J R Knox
Journal:  Biochemistry       Date:  1997-03-04       Impact factor: 3.162

6.  ATP-dependent inactivation and slow binding inhibition of Salmonella typhimurium D-alanine:D-alanine ligase (ADP) by (aminoalkyl)phosphinate and aminophosphonate analogues of D-alanine.

Authors:  K Duncan; C T Walsh
Journal:  Biochemistry       Date:  1988-05-17       Impact factor: 3.162

7.  D-amino acid aminotransferase of Bacillus sphaericus. Enzymologic and spectrometric properties.

Authors:  K Yonaha; H Misono; T Yamamoto; K Soda
Journal:  J Biol Chem       Date:  1975-09-10       Impact factor: 5.157

8.  D-Alanyl-D-lactate and D-alanyl-D-alanine synthesis by D-alanyl-D-alanine ligase from vancomycin-resistant Leuconostoc mesenteroides. Effects of a phenylalanine 261 to tyrosine mutation.

Authors:  I S Park; C T Walsh
Journal:  J Biol Chem       Date:  1997-04-04       Impact factor: 5.157

9.  Molecular basis for vancomycin resistance in Enterococcus faecium BM4147: biosynthesis of a depsipeptide peptidoglycan precursor by vancomycin resistance proteins VanH and VanA.

Authors:  T D Bugg; G D Wright; S Dutka-Malen; M Arthur; P Courvalin; C T Walsh
Journal:  Biochemistry       Date:  1991-10-29       Impact factor: 3.162

10.  Identification of vancomycin resistance protein VanA as a D-alanine:D-alanine ligase of altered substrate specificity.

Authors:  T D Bugg; S Dutka-Malen; M Arthur; P Courvalin; C T Walsh
Journal:  Biochemistry       Date:  1991-02-26       Impact factor: 3.162
View more
  10 in total

1.  The molecular basis of vancomycin resistance in clinically relevant Enterococci: crystal structure of D-alanyl-D-lactate ligase (VanA).

Authors:  D I Roper; T Huyton; A Vagin; G Dodson
Journal:  Proc Natl Acad Sci U S A       Date:  2000-08-01       Impact factor: 11.205

Review 2.  Vancomycin resistance in enterococci due to synthesis of precursors terminating in D-alanyl-D-serine.

Authors:  Peter E Reynolds; Patrice Courvalin
Journal:  Antimicrob Agents Chemother       Date:  2005-01       Impact factor: 5.191

3.  Structural and functional characterization of VanG D-Ala:D-Ser ligase associated with vancomycin resistance in Enterococcus faecalis.

Authors:  Djalal Meziane-Cherif; Frederick A Saul; Ahmed Haouz; Patrice Courvalin
Journal:  J Biol Chem       Date:  2012-09-11       Impact factor: 5.157

4.  Glycopeptide antibiotic resistance genes in glycopeptide-producing organisms.

Authors:  C G Marshall; I A Lessard; I Park; G D Wright
Journal:  Antimicrob Agents Chemother       Date:  1998-09       Impact factor: 5.191

5.  vanC cluster of vancomycin-resistant Enterococcus gallinarum BM4174.

Authors:  C A Arias; P Courvalin; P E Reynolds
Journal:  Antimicrob Agents Chemother       Date:  2000-06       Impact factor: 5.191

6.  Biochemical and genetic characterization of the vanC-2 vancomycin resistance gene cluster of Enterococcus casseliflavus ATCC 25788.

Authors:  Ireena Dutta; Peter E Reynolds
Journal:  Antimicrob Agents Chemother       Date:  2002-10       Impact factor: 5.191

7.  In vivo characterization of the type A and B vancomycin-resistant enterococci (VRE) VanRS two-component systems in Escherichia coli: a nonpathogenic model for studying the VRE signal transduction pathways.

Authors:  J C Silva; A Haldimann; M K Prahalad; C T Walsh; B L Wanner
Journal:  Proc Natl Acad Sci U S A       Date:  1998-09-29       Impact factor: 11.205

8.  Detection of vanC1 gene transcription in vancomycin-susceptible Enterococcus faecalis.

Authors:  Tiane Martin de Moura; Ana Paula Vaz Cassenego; Fabrício Souza Campos; Andrea Machado Leal Ribeiro; Ana Cláudia Franco; Pedro Alves d'Azevedo; Jeverson Frazzon; Ana Paula Guedes Frazzon
Journal:  Mem Inst Oswaldo Cruz       Date:  2013-06       Impact factor: 2.743

Review 9.  Regulation of Resistance in Vancomycin-Resistant Enterococci: The VanRS Two-Component System.

Authors:  Alexandra A Guffey; Patrick J Loll
Journal:  Microorganisms       Date:  2021-09-25

Review 10.  Recent Advances in the Development of Semisynthetic Glycopeptide Antibiotics: 2014-2022.

Authors:  Emma van Groesen; Paolo Innocenti; Nathaniel I Martin
Journal:  ACS Infect Dis       Date:  2022-07-27       Impact factor: 5.578
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.