Literature DB >> 9275697

Partial purification of smooth muscle cell derived growth factor.

S Yang1, Z Deng, Z Qu.   

Abstract

The serum free medium conditioned by cultured rabbit aortic smooth muscle cells was partially purified using ultrafiltration and heparin affinity chromatography. Incorporation of [3H]-thymidine (3H-TdR) into cell DNA was used to measure the mitogenic activity of the fractions from chromatography for NIH 3T3 fibroblasts. The molecular weight and the iso-electric point of these fractions were determined by NaDodSO4-polyacrylamide gel electrophoresis (SDS-PAGE) and iso-electric focusing, respectively. The results showed that the protein eluted in 1.0-1.6 mol/L NaCl from the heparin-Sepharose was mitogenic for 3T3 cells, and this protein had a molecular weight of 22.8-26.7 ku and an iso-electric point of about 4.6. The fact that the above-mentioned biochemical properties differed from that of PDGF, IGF and FGF suggests that this mitogenic protein may be a separate growth factor.

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Year:  1996        PMID： 9275697     DOI： 10.1007/bf02887962

Source DB:  PubMed          Journal:  J Tongji Med Univ        ISSN： 0257-716X


  10 in total

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Authors:  J Folkman; M Klagsbrun
Journal:  Science       Date:  1987-01-23       Impact factor: 47.728

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Journal:  Atherosclerosis       Date:  1989-07       Impact factor: 5.162

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Journal:  Proc Natl Acad Sci U S A       Date:  1986-04       Impact factor: 11.205

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Authors:  G E Davies; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1970-07       Impact factor: 11.205

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Authors:  B R Oakley; D R Kirsch; N R Morris
Journal:  Anal Biochem       Date:  1980-07-01       Impact factor: 3.365

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Authors:  R Sarzani; P Brecher; A V Chobanian
Journal:  J Clin Invest       Date:  1989-04       Impact factor: 14.808

7.  Insulin-like growth factor I and protein kinase C activation stimulate pulmonary artery smooth muscle cell proliferation through separate but synergistic pathways.

Authors:  E C Dempsey; K R Stenmark; I F McMurtry; R F O'Brien; N F Voelkel; D B Badesch
Journal:  J Cell Physiol       Date:  1990-07       Impact factor: 6.384

Review 8.  The pathogenesis of atherosclerosis: a perspective for the 1990s.

Authors:  R Ross
Journal:  Nature       Date:  1993-04-29       Impact factor: 49.962

9.  Heparin affinity: purification of a tumor-derived capillary endothelial cell growth factor.

Authors:  Y Shing; J Folkman; R Sullivan; C Butterfield; J Murray; M Klagsbrun
Journal:  Science       Date:  1984-03-23       Impact factor: 47.728

10.  Arterial smooth muscle cells express platelet-derived growth factor (PDGF) A chain mRNA, secrete a PDGF-like mitogen, and bind exogenous PDGF in a phenotype- and growth state-dependent manner.

Authors:  M Sjölund; U Hedin; T Sejersen; C H Heldin; J Thyberg
Journal:  J Cell Biol       Date:  1988-02       Impact factor: 10.539
  10 in total

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