Literature DB >> 9260948

Gene cloning and expression and characterization of a toxin-sensitive protein phosphatase from the methanogenic archaeon Methanosarcina thermophila TM-1.

B Solow1, J C Young, P J Kennelly.   

Abstract

With oligonucleotides modelled after conserved regions within the protein-serine/threonine phosphatases (PPs) of the PP1/2A/2B superfamily, the gene for the archaeal protein phosphatase PP1-arch2 was identified, cloned, and sequenced from the methanogenic archaeon Methanosarcina thermophila TM-1. The DNA-derived amino acid sequence of PP1-arch2 exhibited a high degree of sequence identity, 27 to 31%, with members of the PP1/2A/2B superfamily such as PP1-arch1 from Sulfolobus solfataricus, PP1alpha from rats, PP2A from Saccharomyces cerevisiae, and PP2B from humans. The activity of the recombinant PP1-arch2 was sensitive to several naturally occurring microbial toxins known to potently inhibit eucaryal PP1 and PP2A, including microcystin-LR, okadaic acid, tautomycin, and calyculin A.

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Year:  1997        PMID： 9260948      PMCID： PMC179364          DOI： 10.1128/jb.179.16.5072-5075.1997

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  18 in total

Review 1.  Classification of protein-serine/threonine phosphatases: identification and quantitation in cell extracts.

Authors:  P Cohen
Journal:  Methods Enzymol       Date:  1991       Impact factor: 1.600

2.  Isolation and sequence analysis of a cDNA clone encoding a type-1 protein phosphatase catalytic subunit: homology with protein phosphatase 2A.

Authors:  N Berndt; D G Campbell; F B Caudwell; P Cohen; E F da Cruz e Silva; O B da Cruz e Silva; P T Cohen
Journal:  FEBS Lett       Date:  1987-11-02       Impact factor: 4.124

3.  Inhibition of an archaeal protein phosphatase activity by okadaic acid, microcystin-LR, or calyculin A.

Authors:  K A Oxenrider; M E Rasche; M V Thorsteinsson; P J Kennelly
Journal:  FEBS Lett       Date:  1993-10-04       Impact factor: 4.124

Review 4.  Inhibitors of protein kinases and phosphatases.

Authors:  C MacKintosh; R W MacKintosh
Journal:  Trends Biochem Sci       Date:  1994-11       Impact factor: 13.807

5.  Using mismatched primer-template pairs in touchdown PCR.

Authors:  K H Roux
Journal:  Biotechniques       Date:  1994-05       Impact factor: 1.993

6.  A multifunctional prokaryotic protein expression system: overproduction, affinity purification, and selective detection.

Authors:  D J Kroll; H Abdel-Malek Abdel-Hafiz; T Marcell; S Simpson; C Y Chen; A Gutierrez-Hartmann; J W Lustbader; J P Hoeffler
Journal:  DNA Cell Biol       Date:  1993-06       Impact factor: 3.311

7.  Protein phosphorylation in the archaebacterium Sulfolobus acidocaldarius.

Authors:  R Skórko
Journal:  Eur J Biochem       Date:  1984-12-17

8.  A protein-serine phosphatase from the halophilic archaeon Haloferax volcanii.

Authors:  K A Oxenrider; P J Kennelly
Journal:  Biochem Biophys Res Commun       Date:  1993-08-16       Impact factor: 3.575

9.  Identification of a serine/threonine-specific protein phosphatase from the archaebacterium Sulfolobus solfataricus.

Authors:  P J Kennelly; K A Oxenrider; J Leng; J S Cantwell; N Zhao
Journal:  J Biol Chem       Date:  1993-03-25       Impact factor: 5.157

10.  Conservation analysis and structure prediction of the protein serine/threonine phosphatases. Sequence similarity with diadenosine tetraphosphatase from Escherichia coli suggests homology to the protein phosphatases.

Authors:  G J Barton; P T Cohen; D Barford
Journal:  Eur J Biochem       Date:  1994-02-15
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  15 in total

Review 1.  Archaeal protein kinases and protein phosphatases: insights from genomics and biochemistry.

Authors:  Peter J Kennelly
Journal:  Biochem J       Date:  2003-03-01       Impact factor: 3.857

Review 2.  Posttranslational protein modification in Archaea.

Authors:  Jerry Eichler; Michael W W Adams
Journal:  Microbiol Mol Biol Rev       Date:  2005-09       Impact factor: 11.056

3.  Molecular cloning and functional expression of a protein-serine/threonine phosphatase from the hyperthermophilic archaeon Pyrodictium abyssi TAG11.

Authors:  B Mai; G Frey; R V Swanson; E J Mathur; K O Stetter
Journal:  J Bacteriol       Date:  1998-08       Impact factor: 3.490

Review 4.  Eukaryote-like serine/threonine kinases and phosphatases in bacteria.

Authors:  Sandro F F Pereira; Lindsie Goss; Jonathan Dworkin
Journal:  Microbiol Mol Biol Rev       Date:  2011-03       Impact factor: 11.056

5.  The PPP-family protein phosphatases PrpA and PrpB of Salmonella enterica serovar Typhimurium possess distinct biochemical properties.

Authors:  L Shi; D G Kehres; M E Maguire
Journal:  J Bacteriol       Date:  2001-12       Impact factor: 3.490

6.  The archaeon Sulfolobus solfataricus contains a membrane-associated protein kinase activity that preferentially phosphorylates threonine residues in vitro.

Authors:  B H Lower; K M Bischoff; P J Kennelly
Journal:  J Bacteriol       Date:  2000-06       Impact factor: 3.490

7.  The icfG gene cluster of Synechocystis sp. strain PCC 6803 encodes an Rsb/Spo-like protein kinase, protein phosphatase, and two phosphoproteins.

Authors:  L Shi; K M Bischoff; P J Kennelly
Journal:  J Bacteriol       Date:  1999-08       Impact factor: 3.490

Review 8.  Protein Ser/Thr/Tyr phosphorylation in the Archaea.

Authors:  Peter J Kennelly
Journal:  J Biol Chem       Date:  2014-02-19       Impact factor: 5.157

9.  Open reading frame sso2387 from the archaeon Sulfolobus solfataricus encodes a polypeptide with protein-serine kinase activity.

Authors:  Brian H Lower; Peter J Kennelly
Journal:  J Bacteriol       Date:  2003-06       Impact factor: 3.490

10.  PrpE, a PPP protein phosphatase from Bacillus subtilis with unusual substrate specificity.

Authors:  Adam Iwanicki; Anna Herman-Antosiewicz; Marcin Pierechod; Simone J Séror; Michał Obuchowski
Journal:  Biochem J       Date:  2002-09-15       Impact factor: 3.857
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