Literature DB >> 92593

Solid-phase radioimmunoassay for hepatitis Be antigen (HBeAg).

H E Blum, G Dölken, W Gerok.   

Abstract

A solid-phase radioimmunoassay was developed for the detection of HBeAg and anti-HBe in sera or serum fractions. HBe/sAg positive sera, partially purified HBeAg, partially purified HBsAg, and HBe/sAg negative sera were polymerized in polyacrylamide and compared for their ability to bind 125I-IgG (anti-HBe). Only gels containing HBeAg reacted specifically with the iodinated antibody. The specificity of the binding was confirmed by blocking and inhibition tests using anti-HBe, HBeAg, HBsAg, and negative control sera. The radioimmunoassay allows the specific and quantitative detection of HBeAg and anti-HBe even in the presence of detergents and high salt concentrations.

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Year:  1979        PMID： 92593     DOI： 10.1007/bf01481494

Source DB:  PubMed          Journal:  Klin Wochenschr        ISSN： 0023-2173


  17 in total

1.  Preparation of iodine-131 labelled human growth hormone of high specific activity.

Authors:  W M HUNTER; F C GREENWOOD
Journal:  Nature       Date:  1962-05-05       Impact factor: 49.962

2.  A solid-phase radioimmunoassay for Epstein-Barr virus-associated membrane antigen prepared from B95-8 cell culture supernatants.

Authors:  G Dölken; G Klein
Journal:  J Natl Cancer Inst       Date:  1977-05       Impact factor: 13.506

3.  New specificities in Australia antigen positive sera distinct from the Le Bouvier determinants.

Authors:  L O Magnius; J A Espmark
Journal:  J Immunol       Date:  1972-11       Impact factor: 5.422

4.  Protein-containing polyacrylamide gels: their use as immunoadsorbents of high capacity.

Authors:  S Carrel; S Barandun
Journal:  Immunochemistry       Date:  1971-01

5.  Purification and partial characterization of hepatitis e antigen (HBeAg).

Authors:  H A Fields; D W Bradley; C L Davis; J E Maynard
Journal:  Infect Immun       Date:  1978-06       Impact factor: 3.441

6.  A new antigen complex co-occurring with Australia antigen.

Authors:  L O Magnius; A Espmark
Journal:  Acta Pathol Microbiol Scand B Microbiol Immunol       Date:  1972

7.  Type B hepatitis: the infectivity of blood positive for e antigen and DNA polymerase after accidental needlestick exposure.

Authors:  H J Alter; L B Seeff; P M Kaplan; V J McAuliffe; E C Wright; J L Gerin; R H Purcell; P V Holland; H J Zimmerman
Journal:  N Engl J Med       Date:  1976-10-21       Impact factor: 91.245

8.  Detection of HBeAg and anti-HBe in acute hepatitis B by a sensitive radioimmunoassay.

Authors:  G G Frösner; M Brodersen; G Papaevangelou; U Sugg; H Haas; I K Mushahwar; C M Ling; L R Overby; F Deinhardt
Journal:  J Med Virol       Date:  1978       Impact factor: 2.327

9.  Viral hepatitis, type B. Studies on natural history and prevention re-examined.

Authors:  S Krugman; L R Overby; I K Mushahwar; C M Ling; G G Frösner; F Deinhardt
Journal:  N Engl J Med       Date:  1979-01-18       Impact factor: 91.245

10.  Host specificity of a serum marker for hepatitis B: evidence that "e antigen" has the properties of an immunoglobulin.

Authors:  A R Neurath; N Strick
Journal:  Proc Natl Acad Sci U S A       Date:  1977-04       Impact factor: 11.205
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