Literature DB >> 9258437

Cloning and overexpression of polypeptide release factor 1 of Thermus thermophilus.

K Ito1, Y Nakamura.   

Abstract

A prfA gene encoding polypeptide release factor RF1 was cloned from Thermus thermophilus. T thermophilus RF1 shares 68% homology with Escherichia coli RF1, and its overproduction reduced readthrough translation of UAG, not of UGA, in the lacZ gene. Rapid purification of T thermophilus RF1 was achieved by T7-RNA polymerase driven overexpression of T thermophilus RF1 protein with a C-terminal histidine tag.

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Year:  1997        PMID： 9258437     DOI： 10.1016/s0300-9084(97)83516-x

Source DB:  PubMed          Journal:  Biochimie        ISSN： 0300-9084            Impact factor:   4.079


  4 in total

1.  Single amino acid substitution in prokaryote polypeptide release factor 2 permits it to terminate translation at all three stop codons.

Authors:  K Ito; M Uno; Y Nakamura
Journal:  Proc Natl Acad Sci U S A       Date:  1998-07-07       Impact factor: 11.205

2.  Specific bonding of puromycin to full-length protein at the C-terminus.

Authors:  E Miyamoto-Sato; N Nemoto; K Kobayashi; H Yanagawa
Journal:  Nucleic Acids Res       Date:  2000-03-01       Impact factor: 16.971

3.  Chemical engineering of the peptidyl transferase center reveals an important role of the 2'-hydroxyl group of A2451.

Authors:  Matthias D Erlacher; Kathrin Lang; Nisha Shankaran; Brigitte Wotzel; Alexander Hüttenhofer; Ronald Micura; Alexander S Mankin; Norbert Polacek
Journal:  Nucleic Acids Res       Date:  2005-03-14       Impact factor: 16.971

4.  An intact ribose moiety at A2602 of 23S rRNA is key to trigger peptidyl-tRNA hydrolysis during translation termination.

Authors:  Melanie Amort; Brigitte Wotzel; Kamilla Bakowska-Zywicka; Matthias D Erlacher; Ronald Micura; Norbert Polacek
Journal:  Nucleic Acids Res       Date:  2007-07-26       Impact factor: 16.971
  4 in total

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